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《Academic Journal of Second Military Medical University》 2012-02
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Protective effects of Tongluo recipe against injury of glomerular visceral epithelial cells in diabetic rats

SUN Liang-liang1,LI Wen-tong2,FENG Xiao-yun1,ZHANG Lan-yu1,ZOU Jun-jie1,SHI Yong-quan1,LIU Zhi-min1 1.Department of Endocrinology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China 2.The First Sanatorium for Retired Cadre,Garrison Command of Shanghai,Shanghai 200040,China  
Objective To observe the protective effect of α-lipoic acid(ALA) and Tongluo recipe(TLR) against injury of glomerular visceral epithelial cells in diabetic rats,so as to discuss the possible mechanism for injury of glomerular visceral epithelial cells in diabetes.Methods Rat diabetes model was induced by high-fat diet and a single intraperitoneal injection of streptozotocin(35 mg/kg).The diabetic model rats were randomly divided into three groups(n=8): diabetes mellitus(DM) group,DM+TLR and DM+ALA group;another 8 rats served as normal controls(CON group).Rats in the DM+TLR group received 0.4 g·kg-1·d-1 TLR and DM+ALA group received 0.1 g·kg-1·d-1 ALA throughout the experiment.Rats in the DM group and control(CON) group were given celiac perfusion of distilled water once at the same time and volume.After 12 weeks of treatment,kidney mass/body mass index,24 h urinary protein,blood urea nitrogen,and urinary albumin/Cr were determined in each group.The number and the density of podocytes in glomerulis were measured by stereological dissector/fractionator methods.The expression of nephrin in renal cortical tissue was detected by Western blotting analysis.The activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and the content of malondialdehyde(MDA)were measured in the plasma and renal cortical tissues.Results After 12 weeks,the kidney mass/body mass index,24 h urinary protein and urinary albumin/Cr in DM group were significantly increased compared to control group(P0.01);the number and density of podocytes in the glomerulis and nephrin expression in DM group were significantly decreased(P0.01).The kidney mass/body mass index,24 h urinary protein and urinary albumin/Cr in DM+TLR and DM+ALA groups were significantly decreased compared to DM group(P0.01);the number and density of podocytes and nephrin expression in DM+TLR and DM+ALA groups were significantly increased compared to DM group(P0.01,P0.05).GSH-Px and SOD activities were significantly decreased in the plasma(P0.01) and renal cortical tissue(P0.01) in DM,DM+ALA and DM+TLR groups compared to those in the CON group,and the content of MDA was significantly increased(P0.01,P0.05).GSH-Px and SOD activities were significantly increased in the plasma and renal cortical tissue(P both0.01) and MDA content was significantly decreased in DM+TLR and DM+ALA groups(P0.01,P0.05)compared to those in the DM group.No significant differences were found between DM+ALA group and DM+TLR group concerning the above parameters.Conclusion TLR can ameliorate podocyte injury in diabetic rats,which might be associated with its anti-oxidative effect.
【Fund】: 国家重点基础研究发展计划(“973”计划 2005CB523304)~~
【CateGory Index】: R587.24
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【Citations】
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1 Duan Shufang,Ye Zhen Zhejiang Chinese Medical University,Hangzhou(310053);Relationship between Diabetic Vascular Complications and Endodermis Cell Oxidation Stress[J];Journal of Zhejiang University of Traditional Chinese Medicine;2010-06
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4 TANG Wen-jia,ZOU Jun-jie,SHI Yong-quan,XING Yu-wei,LIU Zhi-min Department of Endocrinology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;Effects of Tongluo recipe on renal function and renal expression of CTGF,RAGE,and p-ERK1/2 in rat model of type 1diabetes mellitus[J];Academic Journal of Second Military Medical University;2010-10
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8 SUN Liang-liang1,LI Wen-tong2,WANG Hua3,ZOU Jun-jie1,SHI Yong-quan1,LIU Zhi-min1 1.Department of Endocrinology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China 2.The First Sanatorium for Retired Cadre,Garrison Command of Shanghai,Shanghai 200040,China 3.Department of Cardiology,Wuhan General Hospital,PLA Guangzhou Military Area Command,Wuhan 430070,Hubei,China;Effects of Tongluo recipe on injury of glomerular visceral epithelial cells in streptozotocin-induced diabetic rats[J];Academic Journal of Second Military Medical University;2011-09
9 GUAN Qi-Gang,ZENG Ding-Yin,SUN Xi-Zhuo,ZHOU Xu-Chen,CHENG Ying,MIAO Zhi-Lin,HE Xue-Zhi,HAN Feng-Tong,and ZHANG Li (Department of Cardiology,First Affiliated Hospital of China Medical University,Shenyang 110001,China);Tongxinluo Inhibiting Coronary Artery Early Inflammation Response and Intima Proliferation Induced by Interleukin-1β in Small Swine[J];Chinese Journal of Arteriosclerosis;2007-08
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