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《Academic Journal of Second Military Medical University》 2012-02
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Expression of microRNAs targeting heparinase in gastric cancer

ZHANG Yan1,YU Ji-yao2,GUO Shi-wei3,WANG Yu-hong1 1.Department of General Surgery,Clinical College of Navy Medicine,Second Military Medical University,Beijing 100048,China 2.Department of Pathology,Clinical College of Navy Medicine,Second Military Medical University,Beijing 100048,China 3.Department of General Surgery,Changhai Hospital,Second Military Medical University,Shanghai 200433,China  
Objective To investigate the expression of heparinase(HPA) and miRNAs that may regulate HPA expression in the gastric cancer tissue.Methods The expression levels of HPA were determined by real-time PCR in the gastric cancer tissues.Based on the binding of miRNAs with the 3′ untranslated region(3′-UTR) of HPA,we screened for miRNAs targeting HPA using bioinformatics method and determined the expression levels of these miRNAs in the gastric cancer tissues using real-time PCR.Results The expression of HPA was higher in the gastric cancer tissues compared with those in the tumor-adjacent and normal tissues.Bioinformatics analysis showed that miR-18b,miR-137,miR-502,and miR-299-3p might inhibit HPA expression by binding to its 3′-UTR.Real-time PCR analysis revealed lower expression levels of miR-18b,miR-137,miR-502,and miR-299-3p in the gastric cancer tissues compared with in the adjacent and normal tissues.Conclusion HPA is highly expressed in the gastric cancer tissues;miR-18b,miR-137,miR-502 and miR-299-3p,which may target HPA,are lowly expressed,indicating miRNAs targeting HPA may participate in the carcinogenesis of gastric cancer.
【CateGory Index】: R735.2
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