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《Academic Journal of Second Military Medical University》 2012-02
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Observation of anesthetic effect in abdominal zipper model with miniature pigs

YU Chen-lin,LIU Zhi-xue,TANG Qiu,MA Lei,CAI Li-ping,XU Chen,CUI Shu-fang﹡ Laboratory Animal Center,The Training Department,Second Military Medical University,Shanghai 200433,China  
Objective To study the anesthetic effect of Sumianxin Ⅱ combined with pentobarbital sodium on the abdominal zipper model with miniature pigs,so as to explore a safe and reliable anesthetic method for establishing abdominal zipper model with miniature pigs.Methods Abdominal zipper models were induced with 12 miniature pigs by anesthesia with Sumianxin Ⅱ(0.1 ml/kg,im) and pentobarbital sodium(0.2 ml/kg,iv).The physiological parameter,eyelid reflex,corneal reflex,anal reflex,sedation effect,analgesic effect,muscle relaxant effect,and anesthesia duration were all observed.Results The eyelid reflex,corneal reflex and anal reflex of the 12 miniature pigs disappeared 15 min after intravenous anesthesia;and all the animals were in a good sedation,analgesia and muscle relaxation state,which maintained for 75 min.The induction period of anesthesia was(6.22±0.84) min,anesthesia duration was(79.9±3.6) min,and analepsia period was(66.1±3.7) min,providing a satisfactory anesthesia period of more than 80 min.Conclusion Intravenous Sumianxin Ⅱcombined with intramuscular pentobarbital sodium is a simple,safe,effective and reliable method for establishing abdominal zipper model with miniature pigs.
【Fund】: 上海市科技发展基金(08140901400 09140903100)~~
【CateGory Index】: R-332
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