Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Academic Journal of Second Military Medical University》 2017-02
Add to Favorite Get Latest Update

Association of lovastatin overcoming gefitinib resistance with integrin β1 and Survivin

JI Ze;PAN Xing;NI Dian-tao;SHANG Yan;BAI Chong;Department of Respiratory Medicine,Suzhou Kowloon Hospital,Shanghai Jiaotong University School of Medicine;Department of Nursing,Suzhou Kowloon Hospital,Shanghai Jiaotong University School of Medicine;Department of Respiratory and Critical Care Medicine,Changhai Hospital,Second Military Medical University;  
Objective To investigate the association of lovastatin overcoming gefitinib resistance with the levels of integrinβ1and Survivin in human non-small cell lung cancer(NSCLC)cell line PC9 in vitro,and to explore the possible mechanism.Methods The NSCLC cell line PC9 with acquired gefitinib-resistance were divided into 4groups:control group(RPMI 1640),gefitinib group(1μmol/L gefitinib),lovastatin group(5μmol/L lovastatin),and gefitinib combined with lovastatin group(1μmol/L gefitinib+5μmol/L lovastatin).After treatment with different drugs in each group,the inhibition of cell proliferation was detected by cell counting kit-8(CCK-8)test,the levels of integrinβ1and Survivin mRNA were detected by PCR,and the expressions of integrinβ1and Survivin protein were detected by Western blotting analysis.Results Compared with the control group,lovastatin group and gefitinib group,lovastatin combined with gefitinib treatment had significantly inhibited proliferation of PC9 cells with acquired gefitinib-resistance(P0.01),and the expressions of integrinβ1,Survivin protein and mRNA in PC9 cells were significantly decreased in the three groups(P0.05,P0.01).Conclusion Mechanisms of lovastatin combined with gefitinib in overcoming gefitinib resistance may be through blocking integrinβ1-p-Akt-Survivin signaling pathway,indicating that the combinationtreatment might be an effective strategy for gefitinib resistance.
【Fund】: 国家自然科学基金(81570020);; 教育部留学回国人员科研启动基金;; 浦东新区科技发展基金(PKJ2016-Y49);; 苏州工业园区孵化项目;; 浙江省公益技术应用研究计划项目(2016C33216)~~
【CateGory Index】: R734.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 TAN Bi-bo;LI Yong;FAN Li-qiao;ZHAO Qun;WANG Dong;LIU Yu;The Third Department of Surgery,the Fourth Affiliated Hospital of Hebei Medical University;;Effect of Vav3 gene inhibition on inhibitor of apoptosis proteins and multidrug resistance in gastric cancer cells[J];第二军医大学学报;2015-02
2 ZHU Li-shuang;WANG Hao;CHEN Jia;LIU Chang;LU Hui-qi;HAN Huan-xing;Translational Medicine Center,Changzheng Hospital,Second Military Medical University;Department of Ophthalmology,Changzheng Hospital,Second Military Medical University;;Ibuprofen suppresses proliferation and metastasis of human non-small cell lung cancer cell line NCI-H460 and breast cancer cell line SKBR3[J];第二军医大学学报;2014-12
3 DENG Qin-fang,XU Jian-fang,SU Bo,ZHAO Yin-min,ZHOU Cai-cun Department of Oncology,Shanghai Pulmonary Hospital,Tongji University School of Medicine,Shanghai 200433,China;Integrin β_1 participates in acquired resistance to gefitinib in non-small cell lung cancer[J];第二军医大学学报;2012-06
Chinese Journal Full-text Database 3 Hits
1 NAN Zhenhua;PAN Linghui;Department of Anesthesiology, Affiliated Cancer Hospital, Guangxi Medical University;;Mechanism of Ibuprofen Regulating Migration and Invasion of Liver Cancer QGY-7703 Cells Through PI3K/Akt/mTOR Signaling Pathway[J];肿瘤防治研究;2016-12
2 SHI Hong-juan;LV Hai-long;LEI Ying;QIN Wen-juan;WANG Bo;WANG Zhuo;XING Guo-qiang;YANG Ren-tan;JIANG Yu-feng;Department of Histology and Embryology,School of Medicine,Shihezi University;Department of Hepatobiliary Surgery,The First Affiliated Hospital,School of Medicine,Shihezi University;;In vitro effects of ibuprofen on Echinococcus granulosus protoscoleces[J];中国病原生物学杂志;2016-03
3 Shu Chang;Tang Ze;Zhang Guanglin;Department of Medical Oncology,Huangshi Central Hospital;;The related molecules expression and multidrug resistance correlation in the HT29 colon cancer cell[J];现代肿瘤医学;2016-03
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 TAN Bi-bo~*,LI Yong,FAN Li-qiao,ZHAO Qun,LIU Yu,ZHAO Xue-feng.* Department of General Surgery,the Fourth Affiliated Hospital,Hebei Medical University,Shijiazhuang 050011,China;Relationship between expressions of apoptosis-related proteins and chemosensitivities in gastric cancer[J];中华实验外科杂志;2012-07
2 ZHANG Xue-lan1,ZHOU Cai-cun2,Jü Li-xia2 1.Department of Respiratory Medicine,Soochow University,Suzhou 215006,China;2.Department of Oncology,Shanghai Pulmonary Hospital,Tongji University,Shanghai 200433,China;The role of up-regulation of integrin β_1 in resistance to gefitinib in non-small cell lung cancer[J];肿瘤;2011-06
3 JU Li-xia,ZHOU Cai-cun,TANG Liang,ZHAO Yin-min,YANG Xiao-jun,SU Bo,MENG Shu-yan,LI Wei,YAN Ling-hua,DING Yong-mei.Department of Oncology,Shanghai Pulmonary Hospital,Tongji University,Shanghai 200433,China;Drug resistance mechanism of non small cell lung cancer PC9/AB2 cell line with acquired drug resistance to gefitinib[J];中华结核和呼吸杂志;2010-05
4 SU Bo,SU Chun-xia,ZHANG Hai-ping,DENG Qin-fang,ZHAO Yin-min,ZHOU Cai-cun (Department of Oncology,Shanghai Pulmonary Disease Hospital Affiliated to Tongji University,Shanghai 200433,China);Selection and establishment of gefitinib-resistant PC9 cell line and its gene expression profile[J];肿瘤;2008-07
5 WANG Lei, ZHANG Gui-Mei, FENG Zuo-Hua Department of Biochemistry and Molecular Biology, Tongji Medical College Huazhong University of Science and Technology, Wuhan 430030, China;Down-regulation of survivin expression reversed multidrug resistance in adriamycin-resistant HL-60/ADR cell line[J];Acta Pharmacologica Sinica;2003-12
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved