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《Academic Journal of Second Military Medical University》 2017-02
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Bone tissue engineering of a composite scaffold simultaneously releasing two kinds of bone morphogenetic proteins

LU Chun-wen;WANG Chao;YUAN Jia-bin;DING Mu-chen;XU Xiao-bo;SHI Zhi-cai;MAO Ning-fang;Department of Orthopaedics,Changhai Hospital,Second Military Medical University;Medical Team,No.65571 Troop of PLA Northern Theater Command;  
Objective To prepare a 3D polymer scaffold capable of simultaneously releasing both bone morphogenetic protein(BMP)-2and BMP-7for osteogenic differentiation of hFOB1.19 cells in bone tissue engineering.Methods The BMP-2and BMP-7 microspheres of poly(glycolic acid-co-lactic acid)-polyethylene glycol(PGLA-PEG)were prepared using an emulsion method,and the 3Dporous scaffold of PGLA-PEG was produced by phase separation.Then in order to obtain composite scaffold loaded with BMPs,the two microspheres were fixed into scaffolds with methylene chloride fumigation technology,and the slow-release of BMP-2 and BMP-7 was observed.The human osteoblast line cells(hFOB1.19)were seeded on the prepared composite scaffolds for testing cell proliferation and osteogenic differentiation.Results It was showed that the produced 3Dcomposite scaffolds could release the BMP-2and BMP-7gradually.The hFOB1.19 cells grew better on the 3Dcomposite scaffolds than on the traditional scaffolds on day10 of cell culture(P0.01),and the cells had normal morphology.The mRNA levels of alkaline phosphatase,collagen type-Ⅰ,osteocalcin and osteopontin of cells on the 3Dcomposite scaffolds were higher than those on the scaffold without BMPs(P0.05, P0.01). Conclusion We have successfully prepared a 3D polymer scaffold which can simultaneously release BMP-2and BMP-7,and it may be used bone for tissue engineering.
【Fund】: 国家自然科学基金(81272026 8167090274)~~
【CateGory Index】: R318.08;R68
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