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《Academic Journal of Second Military Medical University》 2017-02
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Effect of autophagy on expression of synaptophysin and postsynaptic density material 95 in CA1 hippocampal region of vascular dementia rats

LIU Bin;LIU Jin-xia;MAO Wen-jing;ZHANG Wen-yan;DENG Chun-ying;ZHANG Jin-xia;MA Yuan-yuan;HE Yong-gui;LI Shi-ying;First Department of Neurology,Affiliated Hospital of North China University of Science and Technology;  
Objective To explore the effect of autophagy on the expression of synaptophysin(Syn)and postsynaptic density material 95(PSD-95)in CA1 hippocampal region of vascular dementia rats.Methods A total of 96 healthy male SD rats were randomly divided into sham operation group(Sham group),vascular dementia model group(VD group),autophagy inhibitor 3-methyl adenine preconditioning group(3-MA group)and autophagy agonist rapamycin preconditioning group(Rap group).Each group was further randomized into 4subgroups 1,2,4and 8weeks after model establishment.The rat model of vascular dementia was established using a modified Pulsinelli four-vessel occlusion method.The expressions of Syn and PSD-95 in CA1hippocampal region of the vascular dementia rats were detected by Western blotting analysis.Results(1)Compared with the Sham group,the ratio of LC3-Ⅱ/LC3-Ⅰin CA1 hippocampal region of the vascular dementia rats was significantly increased and the expressions of Syn and PSD-95 were significantly decreased in VD group at different time points(all P0.01).Compared with the VD group,the ratio of LC3-Ⅱ/LC3-Ⅰ was significantly decreased and the expressions of Syn and PSD-95 were significantly increased in 3-MA group at different time points(P0.05 or P0.01);the ratio of LC3-Ⅱ/LC3-Ⅰ was significantly increased and the expression of Syn and PSD-95 were significantly decreased in Rap group at different time points(all P0.01).(2)Correlation analysis showed that the ratio of LC3-Ⅱ/LC3-Ⅰ was negatively correlated with Syn and PSD-95expression(all P0.01).Conclusion Autophagy may inhibit the expression of synaptic plasticity related proteins,Syn and PSD-95,in CA1 hippocampal region of the vascular dementia rats,and suppressing autophagic activity is benefitial to the synaptic plasticity.
【Fund】: 河北省高等学校科学技术研究重点项目(ZH2012046);; 河北省重大医学科研课题(zd2013087)~~
【CateGory Index】: R749.13
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