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《Academic Journal of Second Military Medical University》 2017-12
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Effect of senescence-associated secretory phenotype-conditioned medium on proliferation of human gastric cancer cell lines BGC823

WANG Yu;ZHU Zhen-xin;TANG Yuan;FENG Dan;ZHANG Xin;WANG Chang-ming;CAI Qing-ping;Department of General Surgery (Ⅱ),Changzheng Hospital,Second Military Medical University;  
Objective To investigate the effect of senescence-associated secretory phenotype-conditioned medium(SASP-CM)on the proliferation of human gastric cancer cell lines BGC823.Methods BGC823 cells were divided into three groups:SASP-CM group,tumor cells control-conditioned medium(CTR-CM)group,and normal-conditioned medium(NOR-CM)group.BGC823 cells in the SASP-CM group were treated with paclitaxel(PTX)to establish senescent cell model and to prepare SASP-CM.The establishment of senescent cell model was confirmed by senescenceassociatedβ-galactosidase staining.The concentrations of major SASP factors in SASP-CM were detected by enzyme linked immunosorbent assay,the proliferation ability of BGC823 cells was detected by CCK-8 assay and cell clone formation assay,and the cell cycle and apoptosis were detected by flow cytometry.Results After treating BGC823 cells with 35 nmol/L PTX for 72 h,a steady senescence model was established,which had the highest percentage of senescence cells([66.95±3.54]%).The concentrations of interleukin(IL)-6,IL-8,chemokine(C-X-C motif)ligand 1(CXCL1),chemokine(C-C motif)ligand 2(CCL2),and interferonγ(INF-γ)in the SASP-CM were significantly higher than those in the CTR-CM(all P0.01).The relative clone formation rate of BGC823 cells in the SASP-CM group was significantly higher than those in the CTR-CM group(P0.01)and NOR-CM group(P0.05).The proliferation activity of BGC823 cells in the SASP-CM group was significantly higher than that in the CTR-CM and NOR-CM groups after incubation for 48,72 and 96 h(all P0.05).The percentage of S phase cells in the SASP-CM group was significantly higher than that in the CTR-CM and NOR-CM groups(both P0.01),and there was no significant difference in cell apoptosis rate between groups.Conclusion SASP-CM can promote the proliferation of human gastric cancer cell lines BGC823.
【Fund】: 国家自然科学基金(81372670 81100629 81402359 81602617);; 上海市自然科学基金(16ZR1436800)~~
【CateGory Index】: R735.2
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