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Roles of DNA polymerase β on repair of DNA damaged by γ-rays irradiation

Cai Jianming;Gao jianguo;Zheng Xiulong; Luo Chengji;Cheng Tianmin; Yang Rujun; Zhao Fang;Zhang Limin; Yang Lixi (Division of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, 200433)  
The roles of DNA polymerase β in the repair synthesis of γ-rays irradiated DNA from calf thymus, SMMC-LTNM hepatoma and nude mouse hepatocytes were evaluated, using NEM or d2 TTP as selective inhibitors to DNA polymerase α or β.It was observed that the rate of[3H]-TTP incorporating into calf thymus DNA damaged by 10 Gy γ-rays exposure was much higher(P0.01) than that of the non-irradiated one,when there was some amount of recombinant rat DNA polymerase β in the reaction mixture.We also found that the [3H]-TTP incorporation rate reflecting the DNA repair synthesis increased gradually as the γ-rays absorbed dose rose to 20 Gy for hepatocyte nuclei or to 5 Gy for hepatoma nuclei, and then decreased slowly as the absorbed dose rose higher. These results suggest that DNA polymerase β could participate in DNA repair synthesis in both normal and neoplastic nuclei irradiated by γ-rays and its roles in DNA repair may be related to cell types and absorbed dose.
【Fund】: 国家自然科学基金
【CateGory Index】: Q523
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