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Forskolin augments the effect of glucocorticoids on proliferation and differentiation of a human osteosarcoma cell line by up-regulation of glucocorticoid receptor

Song Liangnian (Department of Pathophysiology, Department of Basic Medicine, Second Military Medical University, Shanghai, 200433)  
The effect of forskolin,an activator of adenylate cyclase, on glucocorticoid-induced modulation of proliferation and differentiation of a human osteasercoma cell line (HOS-8603) was initially studied.It was found that forskolin could significantly augment the inhibitory effect of glucocorticoids on HOS-8603 cell clonal proliferation and enhance the induction of alkaline phosphatase (AKP) activity by glucocorticoids even thouth forskolin alone had no effect. To clarify the mechanism responsible for the augmenting effect of forskolin on glucocorticoid-induced cellular effect on HOS-8603 cells,the effect of forskolin on glucocorticoid receptor(GR) level was further evaluated.It was shown that forskolin could significantly up-regulate GR level in a timedependent manner with no concomitant change in GR binding affinity for glucocorticoids. These results suggest that the augmentation of glucocorticoid effect on HOS-8603 cell proliferation and differentiation by forskolin is achieved by up-regulation of GR.
【CateGory Index】: R977.11
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