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《Dunhuang Research》 2013-01
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The Fungal Community Composition on Mural Paintings in Tomb of Wei and Jin Dynasty, Jiayuguan

WU Fasi 1,2,3 WANG Wanfu 1,2,3,4 HE Dongpeng 1,2,3 CHEN Gangquan 1,2,3 MA Yantian 4 ZHANG Guobin 1,2,3 ZHANG Xiaodong 5 FENG Huyuan 4(1.National Research Center for Conservation of Ancient Wall Paintings,Dunhuang,Gansu 736200;2.Key Laboratory for Conservation of Ancient Wall Paintings and Earthen Ruins in Gansu,Dunhuang,Gansu 736200;3.Key Scientific Research Base of Conservation for Ancient Mural,State Administration for Cultural Heritage,Dunhuang,Gansu 736200;4.School of Life Sciences,Lanzhou University,Lanzhou,Gansu 730000;5.Management Institute for Xincheng Mural Paintings Tomb of Wei and Jin Dynasty,Jiayuguan,Gansu 736200)  
Microbial biodeterioration is one of the major problems for conservation of mural paintings in tomb of the Wei and Jin dynasty,Jiayuguan.The molecular techniques were used to detect and analyze fungal groups on mural paintings in this study.After the extraction of the samples’total DNA,amplification of the targeted fragments,construction of the clone library,and determination of sequences,we found that most of the fungal sequences affliated to the genus Aspergillus,Phialosimplex,and Engyodontium.The diversity of fungi decreased in tomb that was being disturbed because of daily tourists visiting,the community strutures changed simultaneously.Both measures of opening to the public or closing up all the year round are harmful for the conservation of the tomb wall paintings.The molecular techniques have dominant position in the detection of microbial community rapidly and accurately,which can be popularized in the future studies.
【Fund】: 敦煌研究院院级课题“嘉峪关魏晋墓(六号、七号墓)腐蚀壁画微生物群落结构初步研究”(201008);; 世界银行贷款项目“魏晋墓砖壁画保护研究和鲁土司衙门妙音寺壁画保护研究”(GSC-7542-CN);; 国家自然科学基金课题(31070344);; 国家文物局文物保护科学与技术研究重点课题(20110208)
【CateGory Index】: TU-87
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