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Clonning and sequencing of partial gene of TTV from Nanjing in China

Yang Zhiguo,Xu Jiazhang ,Zhou Yusen, et al. Department of Infectious Diseases, The 81st Hospital of PLA, Nanjing,China ,210002.  
To investigate the presence of TTV infection in Nanjing, China. Methods Serum samples from patients ofhepatitis diseases were detected for TTVDNA using PCR method and the PCR product was sequenced. Results TTVDNA was detected in 8 of 14 patients with Non A-E hepatitis. The partial gene was cloned and sequenced from the serum of one patient. The partial sequence of TTV from Nanjing distinguishly showed 97. 65% and 98. 99% nucleotide identity over the corresponding region of TTV isolated in Jpan and in China. Conclusion There are patients with TTV infection in Nanjing,China.
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