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CHANGES OF ISOENZYME PATTERNS OF PEROXIDASE AND EASTERASE DURING EARLY PROCESS OF SOMATIC EMBRYOGENESIS IN SAINFOIN (Onobrychis viciaefolia Scop)

Lu Tie-gang, Wang Yi-shen and Zheng Guo-chang (Laboratory of Cell Bioloyy, Lanzhou University, Lanzhou 730000)  
Hypocotylar explants of Onobrychts viciaefolia Scop were cultured on LS medium containing 1 mg/l BA, 1 mg/l KT. Two weeks after culture calli were initiated on the surface of sections. Nonembryogenic callus but with simitiar physiological state and higher embryogenic capacity was obtained by the selection and proliferation of light-yellow callus formed from one explant, and then it was trans ferred to LS medium containing 1mg/l BA to initiate somatic embryogenesis. Obvious changes of isoenzyme patterns were found. Two marked bands of isoperoxidase disappeared 10 days after embryogenic culture. The activity ratio of each isoeasterase band changed greatly during somatic embryogenesis, the number ofisoeasterase bands reduced during later embryogenie culture. Some isoenzyme bands appeared or became stronger while others disappeared or became weaker during somatic embryogenesis. From the results the author suggested that the expressions of venetic informations had a selective mechanism and were regulated by hormones.
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