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Studies on the induction of embryoid from unpollinated ovaries of Nicotiana tabacum L.

PAN Li 1,YANG Tie zhao 2 (1.Landscape Architectural Bureau of Zhengzhou,Zhengzhou 450006,China) (2.Henan Agricultural University,Zhengzhou 450002,China)  
Induction of embryoid from unpollinate ovaries of Nicotiana tabacum L.was investigated in this thesis.The results obtained were summarized as follows.Embryoid was originated from single cell,either megaspore or egg cell.Ovary inoculated at early stage produced embryoid from megaspore,while ovary inoculated at late stage produced embryoid from egg cell.When ovaries were plated at middle stage,embryoid originated more from megaspore than from egg cell.The ability of embryoid production of various genotypes,various concentrations of sucrose,various light intensity changed greatly.
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