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《Acta Botanica Boreali-occidentalia Sinica》 2004-09
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Cytological studies on meiosis and male gametophyte development in cucumber

CAO Qing-he,CHEN Jin-feng~*,QIAN Chun-tao,GUO Jun-yang ticulture,Nanjing Agricultural University,Nanjing 210095,China)  
Southern and Northern Chinese cucumbers were used to study meiosis and male gametophyte development using improved staining and chromosome preparing methods.The results obtained included:① the meiosis in PMCs of cucumber was simultaneous cytokinesis.Chiasmata could be seen clearly in diplotene and the chromosomes could be identified as rod or ring bivalents.The multi-nucleoli and the nucleoli fusion were observed at both telophase Ⅰand telophase Ⅱ;② The microspores in tetrad stage showed the arrangement of decussate type instead of isobilateral type;③ Two nucleuses were centrally placed in male gametophyte development.Only one layer of pollen wall was developed during the centrally placed nucleus stage,and two layers of pollen wall were in side-placed nucleus stage;④ Mature pollen grains were bicellular.
【Fund】: 国家高技术研究发展计划专项经费(2002AA241251;2002AA207012);; 国家自然科学基金(30170644);; 教育部《跨世纪优秀人才培养计划》;; 教育部科技研究重点项目资助课题(01097)
【CateGory Index】: S642.2
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