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《Acta Botanica Boreali-Occidentalia Sinica》 2017-07
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Effects of Ultra-dry on the Resistance Related Physiological and Biochemical Indexes in Seed of Populus euphratica

LIU Yanping;LI Limin;ZHOU Xiaoming;ZHOU Dengpan;ZHANG Li;College of Plant Science,Tarim University/Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin;College of Life Science and Technology,Shenyang Agricultural University;  
The ultra-dry preservation of the rare and endangered species Populus euphratica seeds was carried out to determine the optimal moisture content and rewetting method in this paper.The feasibility of ultra-dry preservation of P.euphraticaseeds was determined by measurements of contents of various enzymes,soluble sugar and malondialdehyde(MDA).The P.euphraticaseeds with different water contents were prepared by the method of dehydration of silica gel.The seeds were re-wetted with different solutions,and the optimal wetting method was selected.After treated by aging,the activities of enzymes,soluble sugar,and malondialdehyde in the optimum moisture content of seeds were determined after rewetting to determine the effect of ultra-dry preservation.The results showed that:(1)the seed germination ratedecreased with the water content decreased to 3.4%.The seed germination rate and germination potential of P.euphraticaseeds could be restored to the samples without ultra-dry treatment after re-wetting.The optimum moisture content was determined as 3.4%.(2)After comparison of four different methods,the results showed that re-wetting with saturated NH4 Cl for 12 hafter re-wetting with saturated CaCl2 was a better method of re-wetting.(3)The aging results showed that the suitable ultra-dry treatment could increase the activities of peroxidase,catalase,dehydrogenase and the content of soluble sugar,and decrease the content of MDA in the seeds with 3.4% moisture at 50℃.Therefore,ultra-dry preservation of P.euphraticaseeds has a certain feasibility for enhancing the anti-aging ability of the seeds.
【Key Words】: Populus euphratica seed ultra-dry preservation physiological and biochemical indexes antiaging ability
【Fund】: 国家自然科学基金(31160069)
【CateGory Index】: S792.11
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