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《Journal of Northeast Petroleum University》 2017-06
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Analysis of microbial diversity and functional strain screening evaluation from Liaohe oilfield sludge

Chen Qing;Cheng Fang;Song Xiaofeng;Lin Jiawei;Dong Yang;He Wei;Zhao Jing;Dalian Chivy Biotechnology Company Limited;State Key Laboratory of Fine Chemicals,Dalian University of Technology;Department of Biomedical Engineering,Nanjing University of Aeronautics and Astronautics;  
On the basis of analyzing the diversity,dominant microflora and distribution characteristics of endogenous microbial species in oily sludge samples in Liaohe oilfield,the feasibility of application of bioremediation in oily sludge was discussed. Three groups of oily sludge samples were collected in Liaohe oil field. The next generation sequencing technique with high throughput was adopted to determine the 16 S rD NA V3 V4 to analyze the abundance,distribution and diversity of species and the discrepancy of microbial species abundance in different samples. The results showed that the numbers of microbial groups in genus level were 51,135 and 213,respectively. The classification and proportion of microbes has significant differences. Among the dominant bacteria,several oil-degradation functional microorganisms are screened,such as Bacillus and Nocardiopsis. LH 03 was screened from the sludge and identified as Bacillus subtilis,its fermented liquid has low surface tension( 29. 3 mN/m) and high emulsifying property on kerosene( 6. 1 cm) and liquid paraffin( 5. 7 cm). Compound bacteria agent of LH 03 and Pseudomonas aeruginosa CB 01 has the ability to degrade oil of 51. 9%,paraffin of 38. 9%and enhanced oil recovery of 12. 7% in sand pack model with the indoor experiments. The bioinformatics analysis clarifies that Liaohe oilfield sludge contains rich material basis of microbial remediation,and has the feasibility of in situ bioremediation through bio-stimulation and enhancement technology.
【Fund】: 辽宁省“百千万人才工程”重点项目(2015-4);; 大连市科技计划项目(2015F11GH089)
【CateGory Index】: Q938;TE357.9
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