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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Influential factors of piezoelectric quartz crystal sensor in liquid phase

ZHANG Bo,FU Wei ling,JIANG Tian lun,CHEN Ming,YU Fan,CHEN Xiao bing (Department of Clinical Laboratories,Southwest Hospital,Third Military Medical University,Chongqing 400038,China)  
Objective To investigate the influential factors in the determination with piezoelectric quartz crystal sensor in liquid phase. Methods A quartz crystal microarray (2×5), 10 MHz quartz AT cut with gold coated electrodes was made. Frequency changes were evaluated in glycerol PBS (0.15 mol/L, pH 7.0)at different viscosities and densities,NaCl at varied concentrations, phosphate solution (0.067 mol/L) at differing pH values,PBS buffer (0.15 mol/L, pH 7.0)at varying volumes respectively. And,the effects of repeatedly impacting and adding solution on the frequency of the crystal were estimated. Results The oscillation frequency was decreased in accordance with the increase of viscosity,density,ion concentration and pH value. The data showed that the volume of PBS, repeatedly impacting and adding solution had no influence on the frequency. Conclusion The characteristics of the contacting solution such as viscosity,density,concentration of ion and pH value markedly interfere the oscillation frequency of quartz crystal markedly. Attention should be paid on these factors in biological determination.
【Fund】: 国家“九五”科技攻关项目 (96 A2 3 0 4 0 4 );; 国家自然科学基金资助面上项目 (3 9870 83 1);; 国家高技术计划生物技术领域青年基金资助项目 (“863”项目 );; 全军“九五”医学科研计划资助面上项目(98MO94 );; 全军“十五”医学科研计划资助面上项目 (0 1MA180 )
【CateGory Index】: R197.39
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