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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Effects of ionic strength on kinetic parameters of hybridization with piezoelectric gene sensor arrays

LIU Ming hua, FU Wei ling, ZHANG Xue, CHEN Ming, XUE Qiang (Department of Clinical Laboratories, Southwest Hospital, Third Military Medical University, Chongqing 400038, China)  
Objective To investigate the effects of ionic strength on kinetic parameters of hybridization with piezoelectric gene sensor arrays. Methods A gene sensor array of 2×5 was made with gene probes of drug resistant genes from Staphylococcus aureaus immobilized on gold coated surfaces . The complementary sequences of these genes were hybridized on the gene sensor in 5 kinds of Tris HCl buffer solutions containing NaCl at different concentrations. The data of sensor frequency were collected and the kinetic parameters analyzed with computer. The hybridization time and frequency changes were compared at different NaCl concentrations. Results With the increase of NaCl concentration, binding rate constant, association constant, maximum binding amount and the frequency differences between before and after hybridization were all increased, but dissociation constant was decreased. Meanwhile, the range of frequency fluctuation was extended and the time for hybridization prolonged along with the increase of NaCl concentration. Conclusion Proper ionic strength is beneficial for gene hybridization, but high ionic strength is harmful to the oscillation of piezoelectric crystal.
【Fund】： 国家“九五”科技攻关项目 ( 96 A2 3 0 4 0 4 );; 国家自然科学基金资助面上项目 ( 39870 831 );; 国家高技术计划生物技术领域青年基金资助项目 (“86 3”项目 ;; 全军“九五”医学科研计划资助面上项目( 98MO94 ;; 全军“十五”医学科研计划资助面上项目 ( 0 1MA1
【CateGory Index】： R446.9
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【References】
Chinese Journal Full-text Database 1 Hits
1 CHEN Ming, FU Wei-ling, CAI Guo-ru, LIU Ming-hua, ZHANG Bo, CHEN Qing-hai, YU Fan (Department of Laboratory Medicine, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China);Construction of A New-style Detection System of Peptide Nucleic Acid Gene Sensors Array[J];Chinese Journal of Nosoconmiology;2004-01
【Citations】
Chinese Journal Full-text Database 1 Hits
1 LIU Ming hua, FU Wei ling, WANG Song lin, HUANG Qing, WANG Jiang hua (Department of Laboratory Diagnosis, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Construction of piezoelectric gene sensor arrays[J];Acta Academiae Medicine Militaris Tertiae;2001-07
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Xin 1\ \ ZHOU Kangyuan 1\ \ GU Yu 1\ \ KE Wenhong 2 1. University of Science and Technology of China, Hefei 230027, P.R.China; 2. 105 Hospital of PLA, Hefei 230031, P.R.China;The Development and Applications of Piezoelectric Biosensors[J];Journal of Transcluction Technology;2003-03
2 WANG Xiao-rui1,2, ZHANG Yun2, ZHANG Yu-wei2, WANG Hua2(1.Department of Applied Chemistry, Hunan Chemical Industry Vocation Technology Institute, Zhuzhou Hunan 412004,China;2.State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College,Hunan University, Changsha 410082,China);A Piezoelectric Schistosoma-Japonicum Immunosensor Based on Self-Assembled Cystamine Film and SiO_2 Nanoparticles[J];Chinese Journal of Sensors and Actuators;2009-02
3 ZHANG-Bo 1, FU Wei-ling 1, TANG Wan-li 2 (1.Southwest Hospital , Third Military Medical University,Chongqing 400038,China; 2.Dept of Bioengineering,China Jialing Group,Chongqing 400032,China);Development of piezoelectric quartz crystal biosensor's application research[J];Journal of Transducer Technology;2002-05
4 LI Wen-peng1, YE Lin2, KANG Qi1, SHEN Da-zhong1(1.Sch of Chemistry and Chemical Engin,Shandong University,Jinan 250100,China;2.Beijing Institute of Radio Metrology & Measurement,Beijing 100830,China);Determination of microcontent water in organic solvents by lithium tetraborate crystal resonant sensor[J];Journal of Transducer Technology;2004-09
5 ZHANG Ping, FENG Li-shun, KANG Qi, SHEN Da-zhong (Sch of Chem and Chem Engin,Shandong University,Jinan 250100,China);Monitor adsorption of surfactant on PVC film by piezoelectric sensor[J];Journal of Transducer Technology;2005-04
6 QI Yong,KANG Qi,LI Xiao-yu,SHEN Da-zhong(School of Chemistry Chemical Engineering and Material Science,Shandong Normal University,Jinan 250014,China);Monitor adsorption performance of surfactant on paraffine film by LCM[J];Transducer and Microsystem Technologies;2006-09
7 LIU Ming hua,FU Wei ling,CHEN Ming,et al. (Department of Medical Laboratory Science,Southwest Hospital,Third Military Medical University,Chongqing 400038);Effects of temperature on hybridization efficiency in piezoelectric gene sensor arrays[J];Chongqing Medical Journal;2001-06
8 ZHANG Bo,FU Wei ling,MAO Qiong guo,et al. (Department of Clinical Laboratories,Southwest Hospital,the Third Military Medical University,Chongqing 400038,China);Establishment and applications of the platform for detecting oscillation frequency of piezoelectric quartz crystals[J];Chongqing Medical Journal;2003-12
9 WANG Xue-ying, SUN Ming-wei, GU Feng, WU Jin-nan, YIN Fan (School of Chemistry and Material Engineering, Changshu Institute of Technology, Changshu 215500, China);Rapid Detection of Total Bacteria Content in Milk Based on Piezoelectric Technique[J];Journal of Changshu Institute of Technology;2011-10
10 LIU Ming hua, FU Wei ling, WANG Song lin, HUANG Qing, WANG Jiang hua (Department of Laboratory Diagnosis, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Construction of piezoelectric gene sensor arrays[J];Acta Academiae Medicine Militaris Tertiae;2001-07
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 TIAN Ning, HUANG Shan, ZHANG Jian (2nd People's Hospital of Wuhu City, Anhui Province,Wuhu 241000);The progress of auto mated coagulation analyzer in method and technology[J];Anhui Medical and Pharmaceutical Journal;2001-04
2 Guo Aiyan, Wang Yinzhang, Huang Zhaojian,et al.(Department of Endocrinology,PLA General Hospital, Beijing 100853);Determination of Serum Insulin by Solid Phase Radioimmunoassay with Ab_2-immobilized Tubes[J];LABELED IMMUNOASSAYS AND CLINICAL MEDICINE;1994-02
3 Yong Guidong Guan Pingfeng(Department of Precision Instruments and Mechanology, Tsinghua University, Beijing 100084);On the Problems in investigation of Acoustic Load Sensitivityof Quartz Crystal Resonator and Related Sensors[J];Journal of Transcluction Technology;1996-04
4 YE Wei-quan 1, ZHOU Kang-yuan 1, WANG Jun 1, CHEN Xin 1, LIU Sheng 1 FU Shao-jun 2, TAO Xiao-ming 2, HUO Tong-lin 2, HONG Yi-lin 2, XU Xiang-dong 2, ZHOU Hong-jun 2 (1. Dept. of Elec. Engin. and Info. Sci., University of Science and;A study on the quartz micro-biosensors[J];JOURNAL OF TRANSDUCER TECHNOLOGY;2000-04
5 Fu Hongqiao Wen Yumei Li Ping (Department of Optoelectronic Information Engineering,Chongqing University,Chongqing 400044);Data Acquiring and Processing on Quartz Resonance Weighing Sensor[J];JOURNAL OF TRANSDUCER TECHNOLOGY;1998-05
6 Xue YanhuiKang Qi (Department of Globe Resources,Shangdong Institute of Mining and Technology,Taian 270019 ) Shen DazhongChen Lingxin (Chemistry College ,Shangdong University);Adsorption of Protein onto Self-Assembled Monolayer In Situ Monitored by Using a Piezoelectric Sensor[J];JOURNAL OF TRANSDUCER TECHNOLOGY;1999-03
7 ;Research Progress and Outlook of Quartz Crystal Microbalance[J];Sensor World;2000-03
8 LIU Ming hua,FU Wei ling,CHEN Ming,et al. (Department of Medical Laboratory Science,Southwest Hospital,Third Military Medical University,Chongqing 400038);Effects of temperature on hybridization efficiency in piezoelectric gene sensor arrays[J];Chongqing Medical Journal;2001-06
9 CHEN Ming,FU Wei ling,LIU Ming hua,et al (Department of Clinical Laboratories,Southwest Hospital,the Third Military Medical University,Chongqing 400038,China);Effects of ion concentrations on hybridization efficiency with PNA piezoelectric gene sensor[J];Chongqing Medical Journal;2003-12
10 ZHANG Bo,FU Wei ling,MAO Qiong guo,et al. (Department of Clinical Laboratories,Southwest Hospital,the Third Military Medical University,Chongqing 400038,China);Establishment and applications of the platform for detecting oscillation frequency of piezoelectric quartz crystals[J];Chongqing Medical Journal;2003-12
【Secondary References】
Chinese Journal Full-text Database 6 Hits
1 WANG Yi-Qian;XU Qiong;GUO Yang;SHEN Wei-Jian;WU Fu-Ping;SHAO Jing-Dong;Jiangsu Entry-Exit Inspection and Quarantine Bureau;Jiangsu Liang Feng Food Group Co.Ltd;Zhangjiagang entry-exit inspection and quarantine bureau;;Rapid screening of peanut allergen by piezoelectric immunosensing technique[J];Journal of Food Safety & Quality;2014-04
2 ZHAO Xin1,LI Zhen1,GU Da-yong2(1.Coll.of Submarine Navy PLA,Qingdao 266071;2.Shenzhen Graduate Sch.Tsinghua Univ.Shenzhen 518055);Advances in Peptide Nucleic Acid Probe Technology and Its Application in Microbial Detection[J];Journal of Microbiology;2008-01
3 CHEN Ming, FU Wei-ling, WU Rong, CAI Guo-ru, XU Shi-jun, LIU Ming-hua, CHEN Qing-hai.Department of Clinical Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China;Research of new style biological signal amplification system of peptide nucleic acid piezoelectric gene sensor[J];Chinese Journal of Laboratory Medicine;2005-11
4 LUO Yang, FU Wei-ling,ZHANG Xue, ZHANG Bo, CHEN Ming, HUANG Jun-fu,XIA Han(Southwest Hospital, the Third Military Medical University, Chongqing 400038, China);Establishment of a Novel Antibody Immobilizati on Method on Piezoelectric Quarts Crystal Immunosensor[J];Chinese Journal of Nosoconmiology;2005-04
5 CHEN Ming, FU Wei-ling, CAI Guo-ru, YU Li-li, LIU Ming-hua, CHEN Qing-hai, ZHANG Bo, JIANG Tian-lun, WU Rong, WANG Feng(Department of Clinical Laboratories, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China);Effects of Hepatitis B Virus Target Concentration on New Style Peptide Nucleic Acid Piezoelectric Gene Sensor Array[J];Chinese Journal of Nosoconmiology;2005-04
6 WANG Yun-xia, CHEN Ming, YAO Chun-yan, DING Yi, SHI Jian-feng, CAO Liang, LIU Jun-kang, XU Qi-wang, FU Wei-ling(Southwest Hospital, the Third Military Medical University, Chongqing 400038, China);Leaky Surface Acoustic Wave Biosensor and Its Different Detection Methods[J];Chinese Journal of Nosocomiology;2008-03
China Proceedings of conference Full-text Database 1 Hits
1 CHEN-Ming, FU Wei-ling. Wu Rong, CAI Guo-ru, Xu Shi-jun, LIU Ming-hua, CHEN Qing-hai (Department of Clinical Laboratory, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Research of new style biological signal amplification system of peptide nucleic acid piezoelectric gene sensor[A];[C];2005
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Dong Xiandui;Lu Juntao;Cha Chuansin(Electrochemistry Lab.Dept.of Chemistry,Wuhan University,Wuhan 430072);Self-Assembled Monolayers:Preparation,Properties and Application[J];ELECTROCHEMISTRY;1995-03
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