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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Comparison between 2 probe immobilization methods on the surfaces of piezoelectric gene sensors

CHEN Ming,FU Wei ling,ZHANG Bo,JIANG Tian lun,YU Li li,LIU Ming hua,YU Fan,CHEN Xiao bing (Department of Clinical Laboratories, Southwest Hospital, Third Military Medical University, Chongqing 400038, China)  
Objective To make comparison between the 2 methods for immobilizing probe on the surfaces of piezoelectric gene sensors. Methods Human papilloma virus (HPV) probe was immobilized on the gold surface of gene sensor array with thiol method and biotin avidin method, respectively. The changes of frequency and time cost were compared in reactions with different concentrations of probe and target sequences. Results The sensitivity of 2 methods was comparative. The biotin avidin method took less time to react with target sequence and immobilized more probe, and hence combined more target sequence, but with tedious procedures. While thiol method was simple but much more time costing for reaction. Conclusion The 2 immobilization methods both have their own advantages and disadvantages. Suitable method should be chosen in the practice according to different requests.
【Fund】: 国家“九五”科技攻关项目 ( 96 A2 3 0 4 0 4 );; 国家自然科学基金资助面上项目 ( 39870 831 );; 国家高技术计划生物技术领域青年基金资助项目 (“86 3”项目 ;; 全军“九五”医学科研计划资助面上项目( 98MO94 ;; 全军“十五”医学科研计划资助面上项目 ( 0 1MA1
【CateGory Index】: R446.9
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