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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Detection of methicillin-resistant Staphylococci aureus by piezoelectric gene sensor arrays

LIU Ming hua, FU Wei ling, CHEN Ming, HUANG Jun fu, HUANG Qing (Department of Clinical Laboratories, Southwest Hospital, Third Military Medical University, Chongqing 400038, China)  
Objective To investigate the feasibility of the detection of methicillin resistant Staphylococci aureus (MRSA) by piezoelectric gene sensor arrays. Methods The resistant marker gene mecA and specific gene femA of Staphylococcus aureus were detected in 64 collected strains of staphylococcus and the results were compared with those obtained by PCR and conventional antimicrobial susceptibility test. Results The results by sensor arrays were identical to those by PCR, but had some differences with those by antimicrobial susceptibility test. Among 64 strains of staphylococcus, 2 strains of MRSA had negative mecA gene, 2 strains of methicillin susceptible Staphylococci aureus (MSSA) showed positive to mecA, 1 strain of methicillin resistant coagulase negative staphylococcus (MRCNS) showed negative to mecA, and 2 strains of methicillin susceptible coagulase negative staphylococcus (MSCNS) encoded mecA gene fragment. Conclusion With the piezoelectric gene sensor arrays, mecA and femA gene can be detected at the same time, so the instrument provides a rapid diagnosis for MRSA infection.
【Fund】: 国家“九五”科技攻关项目 ( 96 A2 3 0 4 0 4 );; 国家自然科学基金资助面上项目 ( 39870 831 );; 国家高技术计划生物技术领域青年基金资助项目 (“86 3”项目 ;; 全军“九五”医学科研计划资助面上项目( 98MO94 ;; 全军“十五”医学科研计划资助面上项目 ( 0 1MA1
【CateGory Index】: R446.9
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