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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Sensitivity improvement of piezoelectric gene sensor by strand extension reaction

CHEN Ming,FU Wei ling,LIU Ming hua,ZHANG Bo,JIANG Tian lun,YU Fan,CHEN Xiao bing (Department of Clinical Laboratories, Southwest Hospital, Third Military Medical University, Chongqing 400038, China)  
Objective To improve the sensitivity of piezoelectric gene sensor by increasing its surface load on the basis of the principle of strand extension reaction. Methods mecA and femA probes of Staphylococcus aureus were immobilized seperately on the surface of gene sensor array. Klenow large fragment was added to extend the strand for 8 h in room temperature after the probes were hybridized with corresponding target DNA. Results The frequencies of the gene sensor were reduced by (99.2±8.5) Hz and (192.9±13.3) Hz during the hybridization and extension reaction respectively, when 50 nmol/L single strand mecA was added on the surface of the gene sensor array, while (142.4±11.2) Hz and (222.6 ±16.6) Hz for the femA. Before the strand extension reaction, the sensitivity of the gene sensor array was 0.5 nmol/L, and was improved to 0.05 nmol/L after the reaction. Conclusion The sensitivity of piezoelectric gene sensor array is significantly improved by strand extension reaction. This method can be used in the detection of trace DNA and the analysis of homogeneity of nucleic acid.
【Fund】: 国家“九五”科技攻关项目 ( 96 A2 3 0 4 0 4 );; 国家自然科学基金资助面上项目 ( 39870 831 );; 国家高技术计划生物技术领域青年基金资助项目 (“86 3”项目 ;; 全军“九五”医学科研计划资助面上项目( 98MO94 ;; 全军“十五”医学科研计划资助面上项目 ( 0 1MA1
【CateGory Index】: R446.9
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【Co-citations】
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1 ;Application of CHROMagar medium in rapid identification of yeasts:a report of 2485 strains[J];Academic Journal of Second Military Medical University;2007-03
2 SONG Shi ping, HUANG Chun ji, XIE Xiao zhen (Department of laboratory Medicine, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China);Mobility of pathogenic bacteria and their drug resistance in lower respiratory tract infections[J];Acta Academiae Medicine Militaris Tertiae;2002-01
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