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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Detection of prothrombin time with piezoelectric quartz crystal sensor

ZHANG Bo,FU Wei ling,YANG Xin,JIANG Tian lun,CHEN Ming,YU Fan (Department of Clinical Laboratories,Southwest Hospital,Third Military Medical University,Chongqing 400038,China)  
Objective To establish a piezoelectric quartz crystal sensor to determinate the prothrombin time(PT)of plasma. Methods The quartz crystal sensor was 10 MHz AT cut quartz with silver coated electrodes. The oscillating frequency changes were observed in the course of the coagulation reaction of plasma sample,the start point and the end point of the coagulation reaction were showed by the frequency change tendency. Results After the plasma sample was added into the reaction system,oscillation frequency of the crystal was decreased as a ladder,then rose abruptly and descended gradually to balance. The start of the ladder like frequency decrease was regarded as the start point of coagulation reaction,the turning point of the frequency from rise to decrease as the end point of the reaction. The time between the start point and the end was calculated as PT. It was showed there was a significant correlation between the results from our method and the automatic coagulation analyzer (STAGO, France)(γ=0.97), and the variances within and between batches were 0.78% and 2.38% respectively. Conclusion Our method for determining the prothrombin time of plasma is simple,rapid,specific,accurate and cheap,and it might be a valuable method for PT detection in clinical laboratory.
【Fund】: 国家“九五”科技攻关项目 ( 96 A2 3 0 4 0 4 );; 国家自然科学基金资助面上项目 ( 39870 831 );; 国家高技术计划生物技术领域青年基金资助项目 (“86 3”项目 ;; 全军“九五”医学科研计划资助面上项目( 98MO94 ;; 全军“十五”医学科研计划资助面上项目 ( 0 1MA1
【CateGory Index】: R446.11
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