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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Determination of human chorionic gonadotropin by piezoelectric quartz crystal micro-array immunosensor

ZHANG Bo,FU Wei ling,JIANG Tian lun,CHEN Ming,YU Fan,CHEN Xiao bing (Department of Clinical Laboratories,Southwest Hospital,Third Military Medical University,Chongqing 400038,China)  
Objective To construct a new type of piezoelectric quartz crystal micro array immunosensor for the determination of human chorionic gonadotropin(hCG). Methods The crystal was 10 MHz AT cut quartz with gold coated electrodes. The immobilization of the monoclonal antibody of hCG β onto the surfaces of the gold electrodes was accomplished via protein A. Results Experimental results showed that the piezoelectric immunosensor had good response to hCG,the frequency shifts were linearly dependent on the hCG concentration in the range of 2.5~250 mIU/ml,other hormones such as TSH,LH and FSH, did not interfere in the sensor's frequency. The analytical results from this approach were in satisfactory agreement with those of by fluorescent immunoassay ( P 0.05) with correlation coefficiency of 0.92. Conclusion Piezoelectric micro array immunosensor for determination of hCG is of high sensitivity,good specificity,simple operation,rapid analysis,low cost and real time detection etc. It is suitable for detecting hCG in serum and urine in clinical laboratory.
【Fund】: 国家“九五”科技攻关项目 ( 96 A2 3 0 4 0 4 );; 国家自然科学基金资助面上项目 ( 39870 831 );; 国家高技术计划生物技术领域青年基金资助项目 (“86 3”项目 ;; 全军“九五”医学科研计划资助面上项目( 98MO94 ;; 全军“十五”医学科研计划资助面上项目 ( 0 1MA1
【CateGory Index】: R446.6
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