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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Preparation and application of protein microarray for CD series antibodies

WANG Yan, LIU Xin, MIAO Jing ming, SHEN Da zhong, YANG Meng su (Department of Molecular Biology, Third Military Medical University, Chongqing 400038, China)  
Objective Based on antibody antigen reaction to develop protein microarrays for high throughput, rapid detection of multi proteins on cellular membrane surface. Methods Four types of cell lines, HL 60, K562, NB4 and Jurkat were used in this study. The antibodies for CD series were spotted on the aldehyde slide to form microarrays. The cell membrane proteins were isolated and labeled with FSE fluorescence so that the fluorescence signals could be scanned with chip scanner and analyzed after the proteins were captured by immobilized antibodies. The results from protein microarrays were compared with those with Western blot and cell immunohistochemistry. Results With the protein microarrays, HL 60, Jurkat, NB4 and K562 cells were all proved positive to CD13, the former 3 cell lines also showed CD4 positive, and NB4 and K562 cells had positive reaction with CD38 and CD7 antibodies respectively. All of these were identical with the results by Western blot and cell immunohistochemistry. Conclusion The technique of protein microarray is a rapid, simple, low cost and high throughput approach for detecting the CD series on cell membrane.
【Fund】: 国家“九五”科技攻关项目 ( 96 A2 3 0 4 0 4 );; 国家自然科学基金资助面上项目 ( 39870 831 );; 国家高技术计划生物技术领域青年基金资助项目 (“86 3”项目 ;; 全军“九五”医学科研计划资助面上项目( 98MO94 ;; 全军“十五”医学科研计划资助面上项目 ( 0 1MA1
【CateGory Index】: R394-33
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