Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Academiae Medicine Militaris Tertiae》 2002-01
Add to Favorite Get Latest Update

Screening of differentially expressed genes in rat hearts after hemorrhagic shock with suppression subtractive hybridization

ZHANG Wei, WANG Ying ying, YU Zheng ping, WANG Deng gao (Department of Labour Hygiene, Third Military Medical University, Chongqing 400038, China)  
Objective To investigate genetic clues to reveal the mechanisms of severe heart injuries caused by hemorrhagic shock, especially by hypoxia and ischemia in rats. Methods Hemorrhagic shock rat models were established and total RNA were extracted from rat hearts. After the synthesis of the dscDNA, differentially expressed genes was isolated with suppression subtractive hybridization (SSH). Then the isolated genes were cloned and sequenced. The sequencing results were compared with their counterparts in Genbank to search for their homology. Results Among the 42 obtained positive genes, 25 were highly expressed and 17 lowly. Additionally, 5 new sequences were found. Conclusion SSH is an effective method to isolate differentially expressed genes. Hemorrhagic shock changes the expressions of some genes in rat heart, such as Rat 45S rDNA gene transcription initiation region, Rattus norvegicus cysteine rich protein 61 and Rattus norvegicus serum inducible kinase (Snk). But the relation between these genes and the injuries needs further study.
【CateGory Index】: R346
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 WANG Xiao li 1,LIU Li 2,GUO Li 1,FAN Hong xue 1 (1. Dept.of Toxicology,School of Public Health,Jilin University,Changchun 130021,China; 2. Key Laboratory of Molecular Biology, Airforce Hospital);Isolation of differentially expressed genes from rat brains with high and low tolerance to +Gz[J];Journal of Norman Bethune University of Medical Science;2002-05
2 CHEN Jie, QIAN Gui sheng, HUANG Gui jun, XIONG Wei, LI Jing (Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037,China);Suppression subtractive hybridization identified genes differentially expressed in a human lung adenocarcinoma multidrug resistance cell line[J];Acta Academiae Medicine Militaris Tertiae;2001-02
3 CHEN Jun, FAN Qing Yu, ZHANG Hui Zhong, ZHOU Yong Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China;Isolation of metastasis associated genes in osteosarcoma by SSH method[J];Journal of The Fourth Military Medical University;2003-15
4 ZHANG Shou-Xin 1, WANG Bing-Ang 2, XIAO Wen 1, LI Gai-Li 1, ZHANG Hong-Bin 1 1Department of Neurology, 2Department of Orthopaedics, Third Hospital of Chinese PLA, Baoji 721004, China;Effects of Radix Astragali injection on expressions of glial fibrillary acidic protein in peri-intracerebral hemorrhage foci[J];Journal of The Fourth Military Medical University;2005-01
5 CHEN Dong Hong 1,LI Ping 1,CHENG Jian Feng 1,ZHONG Chong Hua 1,HE Jian Rong 1,TENG Shu Bao 1,HE Ping 2 1Deparment of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, 2Dong Fang Institute of Hepato biliary;Effects of radix bupleuri and glycyrrhizin on the hepatocyte apoptosis in mice[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1998-03
6 CHEN Jie, ZHOU Shu-yun, FENG RuDepartment of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China;Identification of differentially expressed genes in bone marrow CD34+ cells by suppression subtractive hybridization[J];Journal of First Military Medical University;2002-09
7 Pang Zhigong, Wang Baoqi, Wang Congying ( Xi'an Medical University, Xi'an 710061)Huang Hong (Xi'an Pharmaceutical Factory);Distribution and Remainer of Tetrahydropalmatine in Rabbit Viscera[J];Chinese Journal of Analytieal Chemistry;1994-06
8 Li Xinwu, et al Biochemistry Teaching and Reseach Section, Hunan Cpllege of Traditional Chinese Medicine;EFFECT OF KANGSHUAI YANSHOU (KSYS) COMPOUND ON TRANSCRIPTION ACTIVITIES OF YOUNG AND OLD RAT LIVER NUCLEI[J];Journal of Traditional Chinese Medicine University of Hunan;1991-04
9 Wang Chunhua, Cheng Jun, Yan Huiping et al. Laboratory of SARS, You′an Hospital, Capital Medical University, Beijing 100054, China;Suppression subtractive hybridization for cloning of genes transactivated by XTP3 protein[J];Medical Journal of Chinese People's Liberation Army;2005-02
10 JIANG Chonghui,YANG Guangtian,TANG Yan,et al.Department of Emergency,Zhongshan City Peoples Hospital,Zhong Shan 528400,China;Effects of L-tetrahydropalmatine on neuronal apoptosis during cerebral ischemia/reperfusion in rats[J];Journal of Emergenoy Medicine;2001-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved