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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Screening of differentially expressed genes in rat hearts after hemorrhagic shock with suppression subtractive hybridization

ZHANG Wei, WANG Ying ying, YU Zheng ping, WANG Deng gao (Department of Labour Hygiene, Third Military Medical University, Chongqing 400038, China)  
Objective To investigate genetic clues to reveal the mechanisms of severe heart injuries caused by hemorrhagic shock, especially by hypoxia and ischemia in rats. Methods Hemorrhagic shock rat models were established and total RNA were extracted from rat hearts. After the synthesis of the dscDNA, differentially expressed genes was isolated with suppression subtractive hybridization (SSH). Then the isolated genes were cloned and sequenced. The sequencing results were compared with their counterparts in Genbank to search for their homology. Results Among the 42 obtained positive genes, 25 were highly expressed and 17 lowly. Additionally, 5 new sequences were found. Conclusion SSH is an effective method to isolate differentially expressed genes. Hemorrhagic shock changes the expressions of some genes in rat heart, such as Rat 45S rDNA gene transcription initiation region, Rattus norvegicus cysteine rich protein 61 and Rattus norvegicus serum inducible kinase (Snk). But the relation between these genes and the injuries needs further study.
【CateGory Index】: R346
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【Co-references】
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