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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Studies on loss of heterzygosity in retinoblastoma

FU Wei ling, WANG Jiang hua, ZHANG Xiao li, ZHOU Lai xin, LEI Man pin, HUA chuan (Department of Clinical Laboratories, Southwest Hospital, Third Military Medical University, Chongqing 400038, China)  
Objective To identify the specific loci containing suspected genes which might play roles in the onset or progress of retinoblastoma (RB) by investigating the incidence of loss of heterozygosity (LOH) on chromosome 13 and the parental origin of deletion with linkage analysis, and to explore the diagnostic and prognostic significance of LOH at specific loci. Methods Paired blood and RB tumor samples from 16 patients were analyzed with fluorescent PCR for LOH at 14 microsatellite marker loci on chromosome 13. Linkage analyses were also performed to identify the parental origin of deletion. Results Of the 16 patients, 12 had LOH at one or more loci. Three markers, D13S265, D13S263 and D13S153 (within Rb1 ), showed the highest incidence of LOH, i.e. 8/12, 8/12 and 9/12 respectively. Ten of 12 LOH cases showed the preferential loss of paternal alleles. The ages when diagnosis was made in LOH and non LOH cases were 504 and 1 086 d respectively. Conclusions Loci D13S263 (13q14.1 14.2) and D13S265 (13q31 32) might contain some unidentified genes related to the tumorigenesis of RB. Most cases in our study showed the preferential loss of paternal alleles. In addition, the age when diagnosis was made was significantly lower in patients with LOH than in those without LOH, and LOH at D13S263, and D13S265 might be used as one of diagnostic indicators at an early age. More data are needed to confirm our findings.
【CateGory Index】: R730.2
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【References】
Chinese Journal Full-text Database 2 Hits
1 DU Xingwei, ZHANG Weiguo, WANG Yi (Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China);CT diagnosis and differential diagnosis of retinoblastoma[J];Acta Academiae Medicinae Militaris Tertiae;2005-19
2 HUANG Qing*, FU Wei-ling, PENG Zhi-pei, et al.*Department of Clinical Laboratory Centre, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China;Analysis of genetic instability of chromosome 19, 20, 21, 22 and X in retinoblastoma[J];Chinese Journal of Ocular Fundus Diseases;2005-01
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 Bai-Chao Ren, Zhi-Lan Bai, Nai-Xue Sun. Department of Ophthalmology, Department of Radiology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China;Clinical value of CT in the diagnosis of 28 patients with retinoblastoma[J];International Journal of Ophthalmology;2003-03
2 TAO Xiao feng , WEI Rui li, SHI Zeng ru, XIAO Xiang sheng, YU Hong. Department of Radiology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;MRI diagnosis of eyeball diseases[J];Chinese Journal of Radiology;2003-02
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 SUN Ge-xin,GAO Xin,XU Chuan-jie,et al.(Depertment of Radiology,The Second Hospital Affiliated Jilin University,Changchun 130041,Jilin);Comparative Analysis of CT of Retinoblastoma and Their Pathologic Diagnosis[J];Chinese Journal of Misdiagnostics;2006-20
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