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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Biological properties and multipotential differentiation of rat bone marrow-derived mesenchymal stem cells in vitro

ZHOU Jin ming, ZOU Zhong min, GUO Chao hua, HE Ying, ZHANG Yong, WANG Jin, WANG Meng, LUO Cheng ji, CHENG Tian ming (Institute of Combined Injury of PLA, Third Military Medical University, Chongqing 400038, China)  
Objective To establish a method of isolating, culturing and identifying rat bone marrow mesenchymal stem cells (MSCs) in vitro and explore their biological properties in order to provide an experimental foundation for applying MSCs to trauma repair. Methods After the tibias and femurs were dissected from 5 to 6 week old rats, the marrow was flushed out with ice cold IMDM medium. The mononuclear cells of the marrow were obtained with density gradient centrifugation and then plated and cultured in IMDM medium. The cultured cells in vitro were identified with their multipotential differentiation. And the growth curve, adhensive rate, cell cycle and ultrastructures of passaged cells were observed. Results The adherent, fibroblast shaped cells approached confluence in single layer 12~16 d after plating. The cultured MSCs in vitro differentiated into osteoblasts and lipoblasts. The double time of MSCs was about 38 h. More than 90% of subcultured MSCs were adhesive in 12 h. The cell cycle analysis showed that 80% of MSCs was in G0/G1 phase. The ultrastructures of MSCs demonstrated the features of infantile cells. Conclusion The subcultured MSCs possess multipotential differentiation. MSCs show stable growth in vitro , easy survival in the subculture and rapid proliferation in present culture condition. And MSCs also exhibit infantility in its ultrastructures and cell cycle. MSCs may be used in the healing of autogeneous trauma.
【Fund】: 国家重点基础研究发展规划资助项目 (“973”项目 ) (G1 9990 5 4 2 0 5 )
【CateGory Index】: R329.2
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