Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Academiae Medicine Militaris Tertiae》 2002-01
Add to Favorite Get Latest Update

Hyperimmune rabbit serum against multiple antigen peptides corresponding to the hypervariable region 1 of HCV prevents viral infection in primary cultured adult tupaia hepatocytes

MIN Feng, HAO Fei, LIU Bing, LIU Guo dong, WANG Yu ming, SONG Zhi qiang (Department of infectious diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China)  
Objective To investigate whether an anti multiple antigen peptide (MAP) rabbit serum against hypervariable region 1 of HCV can block viral infection in cell culture. Methods Anti MAP rabbit sera were respectively mixed in some series of proportions with the first and second samples of HCV RNA positive serum obtained from same patient but at different time. The liver cells of tupaiae were isolated and cultured in the medium with or without positive serum. The cells and supernatant were harvested at various time points during the culturing periods. Both the plus and minus strands of HCV RNA in infected cells or supernatant were examined with reverse transcription polymerase chain reaction (RT PCR). The HCV NS 3 antigens in infected cells were detected with the monoclonal antibodies of them by using immunohistochemical assay. The minus strand of HCV RNA in infected cells was localized with in situ hybridization. Results When the anti MAP rabbit serum were mixed with the second infectious serum, both the plus and minus strands of HCV RNA were detected in culture cells, and the positive signals of NS 3 antigens were found in the cytoplasm of infected cells. But when the anti MAP rabbit serum was added into the first infectious serum (1∶1, 5∶1 and 10∶1), no strand of HCV RNA was detected in culture cells and no positive signals of NS 3 antigens in the cytoplasm. Conclusion The anti MAP rabbit serum could prevent the cultured cells from being infected by HCV infectious sera.
【Fund】: 国家自然科学基金资助项目 (3 9670 672 )
【CateGory Index】: R512.63
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 2 Hits
1 MIN Feng, HAO Fei, LIU Bin, LIU Guo dong, SONG Zhi qiang, HUANG Ji ming, WANG Yu ming (Institute of Infections Diseases of PLA, Southwest Hospital, Third Military Medical University, Chongqing 400038,China);Preliminary study on the cultivation of Tupaia hepatocytes in vitro[J];Acta Academiae Medicine Militaris Tertiae;2001-06
2 WANG Yonggang; HAO Fei; HUANG Yanping; et al. Department of Infectious Diseases,Southwest Hospital, Third Military Medical College, Chongqing 400038, China;Molecular design and immunoreactivity studies of multiple antigen peptide corresponding to hypervariable region 1 sequence of hepatitis C virus[J];CHINESE JOURNAL OF HEPATOLOGY;2000-01
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 Min Feng, Hao Fei,Wang Sumei 174th Hospital of PLA Xiamen 361003;Prelimiary study on morphologic changes of the infected cells of adult normal Tupaia hepatocytes in primary culture by hepatitis C virus in vitro.[J];Modern Medicine Health;2002-01
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 Feng Min~1, Fei Hao~2, Bing Liu~3, Guo Dong Liu~2, Ji Ming Huang~2 and Tian Lun Jiang~2 1 Department of Infectous Disease, The 174 Hospital of PLA. Xiamen 361003, Fujian Province, China 2 Department of Infectious Disease, Southwest Hospital, The Third Mthtary Medical University, Chongqing 400038. China 3 Department of Infectious Disease. The 520 Hospital of PLA Mianyanq China;Isolation and culture of Tupaia hepatocytes[J];World Chinese Journal of Digestology;2001-06
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Wang Hai-Lin;Yan Ziying;Xia NingShao;Hou Yun-De;Jin Dong-Yan(National Lab of Molecular Virology and Genetic Engineering,Instisute of Virology,CAPM.Beijing 100052);Screening of Linear Antigen Epitopes Using Synthetic Peptides Derived from Structural and Non-structural Proteins of HCV[J];;1996-04
2 WANG Ying_Jie, WANG Yu_Ming and LI Meng_Dong Chinese PLA Centre of Infectious Diseases, Southwest Hospital, Third Military Medical College, Chongqing 630038, China;Morphology and function of cultured human fetal hepatocytes[J];;1997-05
3 Li Qifen;Ding Mingquan;Wang Hong; et al. (Department of Infectious Diseases, Southwest Hospital, Third Military Medical University,Chongqing 630038);The infection of hepatitis D virus in adult tupaia[J];NATIONAL MEDICAL JOURNAL OF CHINA;1995-10
4 Wan Tao,Sun Tao①,Wu Jiajin①.Hospital 402,Beijing 100039.① Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850;THE MULTI-PARAMETER PREDICTION OF POTENTIAL PROTEIN ANTIGENIC DETERMINANTS[J];CHINESE JOURNAL OF IMMUNOLOGY;1997-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved