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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Effect of CCK on proliferation of cholangiocarcinoma cells and expression of P21~(WAF1) and P27~(KIP1) genes

MA Kuan sheng, LUO Kun lun, HE Zhen ping, WANG Shu guang, DONG Jia hong (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China)  
Objective To study the effect of cholecystokinin (CCK) on the growth of cholangiocarcinoma cell line QBC939, and explore the relationship between P21 WAF1 and P27 KIP1 gene expressions and QBC939 cell proliferation. Methods Cell counting and flow cytometry (FLC) were used to investigate cell growth, proliferation and cell cycle of the QBC939 cells after co cultured with CCK. The expressions of P21 WAF1 and P27 KIP1 were determined with immunohistochemical staining. Results The proliferation of QBC939 cells with CCK treatment showed an increase in the percentage of cells at S phase and a concomitant reduction of cells at G 1phase, which could be inhibited by L60. The expression levels of P21 WAF1 and P27 KIP1 genes were markedly down regulated in response to CCK treatment. Conclusion The promotion of CCK to QBC939 cell proliferation may be related to the down regulation of P21 WAF1 and P27 KIP1 genes.
【Fund】: 国家自然科学基金资助项目 (3 9770 72 7)
【CateGory Index】: R735.8
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