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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Spontaneous apoptosis and bcl-2/bax expressions in cholangiocarcinoma and their significance

ZHANG Feng shen, MA Kuan sheng, HE Zhen ping, Dong Jia hong (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China)  
Objective To investigate the spontaneous apoptosis and expressions of bcl 2/bax in cholangiocarcinoma and their significance. Methods TUNEL technique and immunohistochemistry were carried out to determine apoptotic cells in situ and the expressions of bcl 2/bax proteins respectively in 40 specimens of cholangiocarcinoma tissues with pathological confirmation. Results The median of spontaneous apoptotic indices (AIs) was 7.89% in 40 cases of cholangiocarcinomas. The AIs of the cases well/moderately differentiated and without lymph node metastasis were significantly higher than those in cases poorly differentiated and with lymph node metastasis respectively ( P 0.01). bcl 2 protein was expressed in 29/40 (72.5%) of carcinoma samples and 0/12 in paracancerous normal bile ducts, while the positive rate of bax protein was 57.5% (23/40) for the former and 83.3% (10/12) for the latter ( P 0.01). Furthermore, well/moderately differentiated carcinomas more frequently expressed bcl 2 compared with poorly differentiated cases ( P 0.01). Finally, bcl 2 expression was inversely, and bax expression was positively related to AI ( P 0.01). Conclusion Apoptosis mechanism remains in cholangiocarcinoma cells, and the bcl 2 mediated apoptotic pathway plays a role in the occurrence and progression of this carcinoma. AI and bcl 2 expression are useful to predict the malignant grade of cholangiocarcinoma.
【Fund】: 国家自然科学基金资助项目 (3 9670 711)
【CateGory Index】: R735.8
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