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《Acta Academiae Medicine Militaris Tertiae》 2002-01
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Structure and variation of E6 and E7 genes of human papillomavirus type 16 from 1 case of cervical carcinoma in Chongqing

HU Li na,XU Bo,QI Hong bo, REN Hong, GU Mei li (Department of Obstetrics and Gynecology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China)  
Objective To study the sequence and variation of the E6 and E7 genes of human papillomavirus 16 type (HPV16) from cervical carcinoma in Chongqing. Methods PCR was used to amplify HPV16 E6 and E7 genes from a sample of cervical carcinoma obtained by biopsy in Chongqing. E6 and E7 the sequences of genes were analyzed before being inserted into the vector pBKS(+) with molecular cloning. Results HPV16 E6, E7 genes were amplified with PCR and inserted into the vector pBKS(+) successfully. Conclusion The lengths of HPV16 E6 and E7 genes are the same as their counterparts of standard HPV16. The sequence of E6 gene is identical to the E6 sequence of Germany strain of HPV16, but, two point mutations were found in E7 gene.
【Fund】: 重庆市科委、卫生局资助项目
【CateGory Index】: R737.33
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【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 YU Meng ~1,MA Zheng-hai~1,Rexidan NIGMAT~1,LIU Kai-jiang~2,ZHANG Fu-chun~1(1.Key Laboratory of Molecular Biology,College of Life Science and Technology,Urumqi,Xinjiang 830046,China;2.The Affiliated Hospital of Cancer,Xinjiang Medical University,Urumuqi,Xinjiang 830011,China);Human Papillomavirus 16 E7 Oncogene Variants in Xinjiang Uygur Women with Cervical Carcinoma[J];Endemic Diseases Bulletin;2006-03
2 WU Xin Xing, SHENG De Qiao, WANG Yu Zhe, ZHAO Ming, DING Xiao Hua, QIU Xiao Ping, TAN Yun, DAI Tian Li, ZHAO Wen Xian Department of Molecular Biology, Virus Research Institute, Hubei Medical University, Wuhan 430071, China;Expression of HPV16E7-HB gene in vitro and in vivo[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-03
3 LIU Jinhua, YU Juan, XU Beini, YAO Jun, ZUO Zehua, ZHAO Min, WU XinxingVirus Research Institution of Medical College of Wuhan University, Wuhan, 430071, China;Expression and Subcellular Localization of Human Papillomavirus 16 Variant Type E7 Protein in HeLa cells[J];Journal of Medical Molecular Biology;2007-01
4 Zhao Min,Wu Xinxing,Zhao Wenxian Virus Research Institute,Basic Medical College, Hubei Medical University,Wuhan 430071,China;Expression of HPV 16(HB Strain) E7 Gene and Studying of Its Antigenicity[J];JOURNAL OF HUBEI MEDICAL UNIVERSITY;2000-01
5 Wu Xinxing,Zhao Min,Ding Xiaohua,Qiu Xiaoping,Tan Yun,Dai Tianli,Zhao Wenxian(Virus Research Institute,Hubei Medical University,Wuhan 430071);STUDY ON ANTIGENICITY AND IMMUNOGENICITY OF HPV16E7 PROTEIN FROM HUBEI OF CHINA[J];IMMUNOLOGICAL JOURNAL;1998-02
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