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《Acta Academiae Medicinae Militaris Tertiae》 2006-10
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Cloning and function analysis of 5′ end flanking sequence of EOLA1 gene

LIANG Zi-wen1, YANG Zhong-cheng2, CHEN Jian2, CHEN Yu2 (1Department of Endocrinology, 2State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burns, Southwest Hospital, Third Military Medical University, Chongqing 400038, China)  
Objective To construct the β-gal reporter genes containing the 5′-end flanking of endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA1) gene in different sequence lengths and identify the sequence, which regulates the gene expression of EOLA1 by the β-gal analysis system. Methods The target sequences were amplified by the method of genome walker, and were inserted into the upstream of β-gal gene located in the β-gal enhancer vector by the directional clone technique respectively; the regulative sequence was identified by analyzing the β-gal activities of reconstructed plasmid in ECV304 cells. Results The regions, containing 2 659 bp and 1 951 bp upstreaming from exon 1, significantly stimulated the reporter gene activity as compared with that of the β-gal control vector in transfected cells. But the region, containing 361 bp upstreaming from exon 1, did not stimulate the reporter gene activity. Conclusion There is an up-regulative element of gene transcription in the region of -361 to -1 951 bp in EOLA1 gene upstream.
【Fund】: 国家自然科学基金资助项目(30200101 30371468)~~
【CateGory Index】: Q78;Q75
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【References】
Chinese Journal Full-text Database 3 Hits
1 LIANG Zi-wen1,ZHOU Guang-ju1,YANG Zong-cheng2,CHEN Jian2,CHEN Yu2( 1Department of Endocrinology,2Sate Key Laboratory of Trauma,Bums and Combined Injury,Institute of Bums,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Identification of EOLA1 gene promoter sequence[J];Acta Academiae Medicinae Militaris Tertiae;2009-23
2 LIANG Zi-wen1,WANG Qing-liang2,YANG Zong-cheng3,CHEN Bing1,ZHANG Zhong-hui1 (1Department of Endocrinology,2Institute of Pathology,3State Key Laboratory of Trauma,Burns and Combined Injury,Institute of Burns,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Preparation of anti-hEOLA1 antibody and detection of EOLA1 in tumor tissues[J];Acta Academiae Medicinae Militaris Tertiae;2009-24
3 ZHOU Guang-ju1,LIANG Zi-wen1,CHEN Yu2,et al(1.Department of Endocrinology,Southwest Hospital of the Third Military Medical University,Chongqing 400038,China;2.Institute of Burns,Southwest Hospital of the Third Military Medical University,Chongqing 400038,China);Construction of recombinant of luciferase promoter reporter gene of endothelial-overexpressed lipopolysaccharide-associated factor 1[J];Journal of Xinxiang Medical College;2008-03
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Liang Ziwen, Yang Zongcheng, Luo Xiangdong et al . Southwest Hospital, Third Military Medical University, Chongqing 400038, China;CLONING OF A NOVEL HUMAN GENE EOLA1[J];Medical Journal of Chinese People's Liberation Army;2003-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIANG Zi-wen,YANG Zhong-cheng,CHENG Jian,et al.(1.Department of Endocrinology;2.Institute of Burns,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Constructing eukaryotic expressive vector of human novel gene EOLA1[J];Chongqing Medicine;2006-10
2 ;Advances in the researches of the prevention and treatment of postburn early damage[J];Acta Academiae Medicinae Militaris Tertiae;2003-18
3 LIANG Zi wen, YANG Zong cheng, LUO Xiang dong, LIU Yue ming, CAI Wen qin (Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Subcellular localization and in vitro expression of the novel gene EOLA1[J];Acta Academiae Medicinae Militaris Tertiae;2003-18
4 CAI Zhen, LIANG Zi-wen, LUO Xiang-dong, YANG Zong-cheng, SUN Rong-ju (State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burns, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Identification of prokaryotic expression of a human eola1 gene in E. coli[J];Acta Academiae Medicinae Militaris Tertiae;2004-23
5 LIANG Zi-wen1,ZHOU Guang-ju1,YANG Zong-cheng2,CHEN Jian2,CHEN Yu2( 1Department of Endocrinology,2Sate Key Laboratory of Trauma,Bums and Combined Injury,Institute of Bums,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Identification of EOLA1 gene promoter sequence[J];Acta Academiae Medicinae Militaris Tertiae;2009-23
6 WANG Fujun,LENG Huaiming,GUO Jianxiu Editorial Department of Acta Academiae Medicinae Zunyi,563003,Zunyi,Guizhou,China;Thoughts on "false citations" among a series of published articles by a research group[J];Acta Editologica;2011-04
7 Liang Ziwen, Yang Zongcheng, Luo Xiangdong et al. Southwest Hospital, Third Military Medical University, Chongqing 400038, China;Subcellular localization and tissue expression of a novel gene EOLA1[J];Medical Journal of Chinese People's Liberation Army;2004-04
8 Liu Yueming, Yang Zongcheng, Liang Ziwen et al. Southwest Hospital, Third Military Medical University, Chongqing 400038, China;Construction and identification of small interfering RNA expression vector targeting EOLA1 gene[J];Medical Journal of Chinese People's Liberation Army;2004-10
9 Ran Chongfu1,Dou Kefeng1,Liang Ziwen2,et al.1.The Department of Hepaticbiliary Surgery of Xi jing Hospital of the Fourth Military Medical University,Xi'an,Shanxi 710032;2.Southwest Hospital,The Third Military Medical University,Chongqing,400038,China;Study on the changes in the expression of EOLA1 in the grafts under the interference of RNA through portal vein following DA→Lewis liver transplantation[J];Sichuan Medical Journal;2007-06
10 ZHOU Guang-ju1,LIANG Zi-wen1,CHEN Yu2,et al(1.Department of Endocrinology,Southwest Hospital of the Third Military Medical University,Chongqing 400038,China;2.Institute of Burns,Southwest Hospital of the Third Military Medical University,Chongqing 400038,China);Construction of recombinant of luciferase promoter reporter gene of endothelial-overexpressed lipopolysaccharide-associated factor 1[J];Journal of Xinxiang Medical College;2008-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIANG Zi wen, YANG Zong cheng, LUO Xiang dong, LIU Yue ming, CAI Wen qin (Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Subcellular localization and in vitro expression of the novel gene EOLA1[J];Acta Academiae Medicinae Militaris Tertiae;2003-18
2 SU Yong-yue, LIANG Guang-ping, LUO Xiang-dong, LIU Yue-ming, CHEN Yu, CHEN Jian, YANG Zong-cheng (State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burns, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Plasmid-based siRNA suppressed target gene EOLA1 expression[J];Acta Academiae Medicinae Militaris Tertiae;2005-03
3 SUN Hui-qin,GUO De-yu,ZHANG Rong,ZHAO You-guang,YAN Xiao-chu,YU Dong-mei,LIU Feng-xuan(Institute of Pathology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Clinicopathologic and immunohistochemical features of metanephric adenoma[J];Acta Academiae Medicinae Militaris Tertiae;2008-21
4 Liang Ziwen, Yang Zongcheng, Luo Xiangdong et al . Southwest Hospital, Third Military Medical University, Chongqing 400038, China;CLONING OF A NOVEL HUMAN GENE EOLA1[J];Medical Journal of Chinese People's Liberation Army;2003-05
5 Fang Xu1,Yinghua Li1,Yinyin Wang1,Fangli Ren1,Li Liu2,and Zhijie Chang1 1 State Key Laboratory of Biomembrane and Membrane Biotechnology,School of Medicine,Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing 100084,China 2 Department of Microbiology and Etiology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100005,China;Preparation of Anti-hNOK Antibodies and Expression Examination of NOK in the Lung Cancer Tissues[J];Chinese Journal of Biotechnology;2008-03
6 LU Xue-chun1,LOU Fang-ding2,HAN Wei-dong3,YU Li2 (1.Gerentological Department of Hematology;2.Hematological department;3.Molecular department,General Hospital of PLA,Beijing 100853,China);Analysis of cis elements in LRP16 gene promoter region[J];China Journal of Bioinformatics;2009-01
7 ZHOU Guang-ju1,LIANG Zi-wen1,CHEN Yu2,et al(1.Department of Endocrinology,Southwest Hospital of the Third Military Medical University,Chongqing 400038,China;2.Institute of Burns,Southwest Hospital of the Third Military Medical University,Chongqing 400038,China);Construction of recombinant of luciferase promoter reporter gene of endothelial-overexpressed lipopolysaccharide-associated factor 1[J];Journal of Xinxiang Medical College;2008-03
8 LIANG Zi-wen , LUO Xiang-dong and YANG Zong-cheng Burn Institute of Southwest Hospital, Third Military Medical University, Chongqing, 400038, China (Liang ZW, Luo XD and Yang ZC);Cloning of differentially expressed genes following lipopolysaccharide stimulation in human umbilical vein endothelial cells[J];中华创伤杂志(英文版);2003-02
9 CAI Zhen, LIANG Zi-wen, LUO Xiang-dong,YANG Zong-cheng, SUN Rong-ju, SU Yong-yue. Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing 400038, P.R. China Corresponding author: LIANG Zi-wen, Email: liangzw99@yahoo.com.cn, Tel: 023-68765215;Purification of human endothelial overexpressed lipopolysaccharide-associated factor 1 protein[J];Chinese Journal of Burns;2005-05
10 LIANG Zi-wen~1, YANG Zong-cheng~2, LIU Yue-ming~2, CHEN Yu~2, LUO Xiang-dong~2. ~1(Department of Endocrinology, Southwest Hospital, Third Military Medical University, Chongqing, 400038 P. R. China. ); ~2(Institute of Burn Research, Southwest Hospital, Third Military Medical University, State Key Laboratory of Trauma, Burn and Combined Injury, Chongqing, 400038 P.R.China);Identification and characterization of a novel gene EOLA1 stimulating ECV304 cell proliferation[J];Chinese Journal of Medical Genetics;2005-05
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 LIANG Zi-wen1,ZHOU Guang-ju1,YANG Zong-cheng2,CHEN Jian2,CHEN Yu2( 1Department of Endocrinology,2Sate Key Laboratory of Trauma,Bums and Combined Injury,Institute of Bums,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Identification of EOLA1 gene promoter sequence[J];Acta Academiae Medicinae Militaris Tertiae;2009-23
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Liang Ziwen, Luo Xiangdong, Yang Zongcheng. Southwestern Hospital, Third Military Medical University, Chongqing 400038;CLONING OF LIPOPOLYSACCHARIDE RESPONSIVE GENES IN ENDOTHELIAL CELLS BY SUPPRESSION SUBTRACTIVE HYBRIDIZATION[J];Medical Journal of Chinese People's Liberation Army;2002-04
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