Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Academiae Medicinae Militaris Tertiae》 2006-10
Add to Favorite Get Latest Update

Expression of CD46, CD55, and CD59 on primary murine astrocytes and effect of inflammation factors on it

CHEN Xue-dan1, BAI Yun1, XIONG Jia-xiang2, SONG Min1 (1Department of Molecular Genetics, 2Department of Physiology, College of Medicine, Third Military Medical University, Chongqing 400038, China)  
Objective To identify the expression of complement regulatory protein CD46, CD55, and CD59 on primary murine pallium astrocytes and the effect of inflammatory factors on it in order to lay the foundation for studying the complement system in AD. Methods The primary murine astrocytes were cultured and purified. The expression of CD46, CD55, and CD59 on the levels of mRNA and protein was assayed by immunofluorescence before and after the stimulation of LPS and IFN-γ. Results The expression of CD59 mRNA was confirmed, but the expression of CD46 and CD55 was indefinite. There was no significant difference between stimulation and non-stimulation groups. Immunofluorescence results indicated that CD59 was positive, while CD46 and CD55 were weakly positive. Conclusion Protectin CD59 expresses copiously on primary murine astrocytes, which presumably protects astrocytes from the lysis of complement.
【Fund】: 国家自然科学基金海外青年学者合作研究基金资助项目(30228018);; 第三军医大学校管课题(200503)~~
【CateGory Index】: R363
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved