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《Acta Academiae Medicinae Militaris Tertiae》 2006-10
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Role of Ca~(2+) signaling in overexpression of detrusor smooth muscle cell connexin 43 by cyclic stretch in vitro

MIN Yong-zheng, LU Gen-sheng, SONG Bo (Urinary Surgery Research Center,Southwest Hospital,Third Military Medical University,Chongqing 400038, China)  
Objective To investigate the role of Ca 2+ signaling in the overexpression of detrusor smooth muscle cell connexin 43 by cyclic stretch in vitro. Methods The detrusor smooth muscle cells (DSMC) grown on collagen-coated silicone membranes were subjected to cyclic stretch-relaxation. The Cx43 mRNA in DSMC were detected with RT-PCR, and the concentration of intracellular free Ca 2+ in DSMC was measured by confocal microscopy in conjunction with the calcium indicator, Fura-3 (Molecular Probes). Results The overexpression of Cx43 mRNA induced by cyclic stretch was significantly inhibited by EGTA.The increase of intracellular free Ca 2+ induced by stretch was also inhibited completely by EGTA, 61.95% by GdCl3, 29.98% by Nifedipine, 87.98% by Ryanodine and Thapsigargin. Conclusion The stretch-induced Ca 2+ entry, via the Ca 2+-induced Ca 2+ release mechanism, may play an important role in DSMC connexin 43 overexpression induced by cyclic stretch.
【CateGory Index】: R694
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 ;Detrusor excitability and instability[J];Acta Academiae Medicinae Militaris Tertiae;2003-22
2 Gen Sheng LU, Bo SONG, Xi Yu JIN, En Qing XIONG (Department of Urology, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China);Ultrastructural Observation of Detrusor in BPH Patients[J];Acto andrologica Sinica;2002-04
3 ZHOU Feng hai,SONG Bo,JIN Xi yu.Department of Urology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China;Expression of gap junction gene connexin and its protein in detrusor instability[J];Chinese Journal of Experimental Surgery;2003-10
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Xin, PANG Jian, SONG Bo, XIONG En qing, JIN Xi yu (Center for Urology of PLA, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Changes of structures and functions in detrusor hyperreflexia in rats following spinal cord transection[J];Acta Academiae Medicinae Militaris Tertiae;2003-22
2 XIONG En qing, ZHAO Xin hong, SONG Bo (Center for Urology of PLA, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Responses of urinary bladder detrusor to neurotransmitters in aged rats[J];Acta Academiae Medicinae Militaris Tertiae;2003-22
3 ZHAO Xin hong, XIONG En qing, SONG Bo, QIU Jian hong (Center for Urology of PLA, Southwest Hospital, Third Military Medical University Chongqing 400038, China);Changes of urodynamics and detrusor structure of aging rats[J];Acta Academiae Medicinae Militaris Tertiae;2003-22
4 LU Gen sheng, GE Ci, SONG Bo, JIN Xi yu (Center for Urology of PLA, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Changes of excitability and contractility in detrusor instability[J];Acta Academiae Medicinae Militaris Tertiae;2003-24
5 JIA Zhuo-min, LU Gen-sheng, Song Bo, LI Wen-gang, WEN Qian-jun, JIANG Hai-hong (Urinary Surgery Research Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Study of relationship between Rho kinase and unstable urinary bladder in rats[J];Acta Academiae Medicinae Militaris Tertiae;2005-10
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7 ZHANG Yuan-ning 1,2,SONG Bo1,JIN Xi-yu1,ZHANG Gen-fu2,WEN Qian-jun1(1Urinary Surgery Research Center,Southwest Hospital,Chongqing 400038,2Renal Diseases Research Center,Department of Urinary Surgery,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China);Expression of BKcaα1 gene in rat bladder of detrusor instability[J];Acta Academiae Medicinae Militaris Tertiae;2006-17
8 JIANG Hai-hong, WEN Qian-jun, SONG Bo Urinary Surgery Research Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China;Study of myogenic spontaneous contractile activities in the detrusor instability rats[J];中国人民解放军军医大学学报(英文版);2006-04
9 ZHANG Wu-he,SONG Bo,FANG Qiang,et al.(Center for Urology of PLA,Southwest Hospital,Third(Military) Medical University,400038,China);Effect of calcium antagonist on functions of isolated detrusor strip in rats following spinal cord injury[J];Medical Journal of National Defending Forces in Northwest China;2006-02
10 ZHOU Feng-hai,WANG Yang-min,SONG Bo,et al.(Department of Urology,Lanzhou General Hospital,Lanzhou Command,PLA,730050,China);Expression of connexin 43 in detrusor instability[J];Medical Journal of National Defending Forces in Northwest China;2007-03
【Secondary Citations】
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1 Chen Yudong Song Bo Jin Xiyu Zhao Xinqiu Qiu Gongkuo Li Longkun Li Xin Department of Urosurgery,the 252 th Hospital of PLA (Baoding 071000);EXPERIMENTAL STUDY OF VASOACTIVE INTESTINAL PEPTIDE ON AUTOMATIC CONTRACTIBILITY OF ISOLATED DETRUSOR URINAE STRIP OF RATS[J];Journal of Hebei Medical University;2001-05
2 QIU Gongkuo , SONG Bo ,JIN Xiyu,et al. Department of Urology,South Western Hospital.3rd Millitary Medical University,Chongqing 400038,China;Effect of bladder outflow obstruction(BOO) on detrusor excitability、contractility and compliance in rat[J];CHINESE JOURNAL OF UROLOGY;2000-07
3 LI Xin *,SONG Bo,JIN Xiyu, et al, *Department of Urology,Southwestern Hospital,Third Military Medical University,Chongqing 400038,China;Relationship between muscarinic receptor subtypes and detrusor hyperreflexia in spinal cord transected rat[J];Chinese Journal of Urology;2002-08
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