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《Acta Academiae Medicinae Militaris Tertiae》 2006-10
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Culture and identification of muscle-derived stem cells from adult rat

YE Jin, JIN Feng-shuo, CHEN Jin, LIANG Pei-he, ZHANG Ke-qin (Department of Urinary Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China)  
Objective To investigate the method of isolating and purifying skeletal muscle-derived stem cells (MDSCs) from adult rat. Methods The MDSCs from mature female SD rats were isolated by enzyme digestion and the cells were purified with Percoll density gradient centrifugation and differentially adherent culture technique. MDSCs were identified by desmin, CD34, CD45, and Sca-1 immunocytochemistry. Results Highly purified MDSCs were harvested and they showed desmin(+),CD34(+),CD45(-),Sca-1(+) by immunocytochemistry. Conclusion MDSCs can be cultured in vitro and used to tissue engineering as “seed” cells.
【CateGory Index】: R329
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