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《Acta Academiae Medicinae Militaris Tertiae》 2006-10
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Human kidney proximal tubular epithelial cell line expresses paxillin induced by TGF-β_1

HAN Jing, ZHANG Jing (Department of Nephrology, XINqiao Hospital, Third Military Medical University, Chongqing 400037, China)  
Objective To investigate the expression of Paxillin (Pax) in human kidney proximal tubular epithelial cell line (HKC) induced by transforming growth factor β1(TGFβ1). Methods HKC cells cultured in vitro were divided into three groups at random: control group(C): cultured with free serum medium(FSM), TGFβ1-treated groups (T1 and T2): cultured with FSM containing different concentrations of TGFβ1 (T1: 5 ng/ml, T2: 10 ng/ml). After 48-hour treatment, the expression of Pax mRNA and protein was detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical staining in HKC cells respectively. Results In Group C, the expression of Pax mRNA and protein in HKC cells was elementary. However, in T1 and T2 groups , the expression of Pax mRNA and protein in HKC cells was greatly increased, especially in T2 group which was more significantly increased than T1 group (P0.01). Conclusion TGFβ1 can induce the expression of Pax in HKC cells in a dose-dependent manner.
【CateGory Index】: R692
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