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《Acta Academiae Medicinae Militaris Tertiae》 2006-10
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Isolation, culture and identification of microvascular endothelial cells from human ovarian carcinoma

YAN Ping 1, YANG Ying 1, HAN Jing 1, HUANG Jian 2 ( 1Department of Obstetrics and Gynecology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, 2Department of Hepatobiliary Surgery, The People’s Hospital of Mianyang, Mianyang 621000, China)  
Objective To establish a reliable method for isolating and culturing microvascular endothelial cells from human ovarian carcinoma. Methods The tissues of human epithelial ovarian carcinoma were finely minced with scissors, then digested. Mononuclear cells were harvested by Percoll gradient centrifugation. The cells were cultured and identified by immunocytochemical staining and transmission electron microscope. Results Twenty-four hours after inoculation, the cells began to cohere, presented polygonal or fusiform. Eight or ten days later, cells grew as confluent monolayer like cobblestones. Factor Ⅷ related antigen was expressed and W-P body was shown in the cells by transmission electron microscope. Conclusion The microvascular endothelial cells can be obtained in vitro from human ovarian carcinoma with our method.
【Fund】: 第三军医大学校管青年科研基金资助项目(2002D076)~~
【CateGory Index】: R737.31
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 JIANG Xue-feng 1, BIAN Xiu-wu 1, WANG Qing-liang 1, LU Jia-you 2, ZHAO Wen 1, SHI Jing-quan 1 ( 1Institute of Pathology, 2Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Isolation, culture, and in vitro angiogenic characteristics of microvascular endothelial cells from human malignant glioma[J];Acta Academiae Medicinae Militaris Tertiae;2005-04
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 BAI Jia-si 1, BIAN Xiu-wu 1, SHI Jing-quan 1, ZHENG Jiang 2, XIN Rong 1 ( 1Institute of Pathology, 2Central Laboratory, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Formation of tubule-like structure of endothelial cells induced by malignant glioma cells in three-dimensional culture in vitro[J];Acta Academiae Medicinae Militaris Tertiae;2004-22
2 Qian Yong, Gao Jiansheng,Zhang Li Eye Hospital, China Academy of Traditional Chinese Medicine, Beijing 100040, China;The culture of retinal microvessels endothelial cells in vitro[J];JOURNAL OF TRADITIONAL CHINESE OPHTHALMOLOGY;1999-03
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