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《Acta Academiae Medicinae Militaris Tertiae》 2006-13
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Enhanced production of MMP-2,MMP-9 by fibroblast cells from rheumatoid arthritis patients when co-cultured with monocytes of high CD147 expression

WU Zhen-biao,ZHU Ping,LU Ning,SHI Zhan-guo,FAN Chun-mei,WANG Yan-hong(Department of Clinical Immunology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China)  
Objective To study the stimulating effects of CD147 on the production of MMPs by fibroblast cells isolated from rheumatoid arthritis patients.Methods Fibroblast cells were isolated from synovial tissues excised from rheumatoid arthritis patients during synovectomy,and confirmed by detection of CD147,CD68 with flow cytometry(FCM) and vimentin protein by immunostaining(SP).The fibroblast-like synoviocytes(FLS) were co-cultured with THP-1 cells(a cell line of monocytes) with high levels of CD147.The production of MMP-2,MMP-9 by synovial fibroblasts was evaluated by Gelatin zymography and RT-PCR.The expression of CD147 on THP-1 cells and synovial fibroblasts was detected by FCM.Antagonist peptide to CD147 AP-9 was used to study the effects on MMPs production.Results The isolated synovial fibroblasts characterized by negative expression of CD14,CD 68 and positive expression of vimentin protein,expressed low levels of CD147 and high levels of MMP-2 and MMP-9,while THP-1 cells expressed much higher levels of CD147 and lower levels of MMP-2,MMP-9.When the synovial fibroblasts were co-cultured with THP-1,the production of MMP-2,MMP-9 by FLS was enhanced,but when co-cultured with the antagonist peptide(AP-9) of CD147,it was suppressed.Conclusion The ability of MMP-2,MMP-9 production by synovial fibroblasts was enhanced when co-cultured with THP-1 of high levels of CD147,and the enhancement can be suppressed by the antagonist peptide(AP-9) of CD147.
【Fund】: 国家高技术研究发展计划(“863”)资助项目(2003AA215061)~~
【CateGory Index】: R593.22
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