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《Acta Academiae Medicinae Militaris Tertiae》 2006-13
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Transformation and chromosomal instability of human tracheal epithelial cells induced by formaldehyde

ZHANG Gui-zhi~(1),XIONG Wei~(2),LIU Chang-ting~(1),LIU Dong-shan~(3)(~1Department of Respiratory Diseases,General Hospital of PLA,Beijing 100853,~2Department of Respiratory Diseases,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,~3Faculty of Public Health and Family Medicine,Capital University of Medical Sciences,Beijing 100054,China)  
Objective To explore the mechanisms of chemical carcinogenesis and the relationship between malignant transformation and genomic/chromosomal instability of human bronchial epithelial cell line by formaldehyde.Methods BEAS-2B cell line was toxicated by formaldehyde and the LC_(50) of formaldehyde was tested by MTT assay.BEAS-2B cells were induced by formaldehyde at 20% LC_(50),and the transformation of BEAS-2B cells was identified and induced clones were selected.By G-banding technique,the metaphase modal chromosome number,karyotypes and nonrandom structural change of induced cell strains were analyzed.Results The growth rate of BEAS-2B cells induced by formaldehyde was significantly increased.The induced cells showed a trend of infinite proliferation and the anchorage independent growth was seen in 15~(th) generation cells.As compared with the relatively stable pseudodiploid karyotype of BEAS-2B cells,most of induced cells were triploid and/or tetraploid karyotypes and their chromosome 14 had lost two copies of chromosome replaced by the other two.They also had high frequency of unstable aberration(39%),including chromosome loss,endoduplication,translocation,breakage,two and/or three centromeres etc..Conclusion The formaldehyde could lead to progression of malignant transformation and result in genomic instability of human bronchial epithelial cells in vitro.
【Fund】: 国家社会公益研究专项(2001DIA10001-6);; 北京市教委科技面上基金资助项目(KM2006100025007)~~
【CateGory Index】: R730.2
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【References】
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1 ZHANG Cheng1, WU Qing-Chen1, LI Qiang1, ZHU Mao-Xiang2, YANG Zhi-Hua2, PAN Xiu-Jie2, XIE Jun-Hao11Department of Cardiothoracic Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China, 2Institute of Radiation, Medical Academy of Military Medical Sciences, Beijing 100850, China;Chromosome aberration during malignant transformation of human bronchial epithelial cells induced by cigarette smoke condensate[J];Journal of the Fourth Military Medical University;2009-09
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1 XIONG Wei,QIAN Gui sheng HUANG Gui jun (Institute of Pulmonology, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China);A primary study on chromosomal aberration of lung cancer[J];Chongqing Medical Journal;2002-09
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【Secondary References】
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1 HE Chong-xiang,JIANG Zhong-fa,WANG Chun-hong,ZHANG Ben-yan. School of Public Health,Wuhan University,Wuhan 430071,China;Effects of the oxidative damage induced by formaldehyde on A549 cells[J];Journal of Public Health and Preventive Medicine;2009-03
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