Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Academiae Medicinae Militaris Tertiae》 2006-21
Add to Favorite Get Latest Update

Effects of brain mitochondrial uncoupling protein content and activity in rat exposed to hypoxia simulating high altitude on mitochondrial energy synthesis

GAO Pan, LIU Jun-ze, XIA Chen (Department of Pathophysiology and Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, Chongqing 400038, China)  
Objective To observe the changes of rat brain mitochondrial uncoupling protein (UCP) content and activity, and explore the effect of UCP on mitochondrial energy metabolism during hypoxia exposure. Methods Adult SD rats were set randomly into control and hypoxia group (n=8 in each group). The rats of hypoxia group were put into a hypobaric chamber simulating 5000-meter high altitude for 3 days (23 h/d). The brain mitochondria was isolated by centrifugation. Mitochondrial oxidative respiratory function was measured by Clark oxygen electrode. Mitochondrial membrane potential was detected by Rhodamine123 method. The content of adenine nucleotide pool (ATP, ADP, AMP) in mitochondria was measured by high performance liquid chromatography. UCP content and activity were detected by [~ 3 H]-GTP binding method. Results High altitude hypoxia resulted in significant increase of UCP activity and a 2.9-fold rise of UCP content of rats (P0.01). Mitochondrial state 3 respiration, respiratory control rate decreased respectively to 83.1% and 66.7% of control group, while the state 4 respiration increased by 28% of control group (P0.01). From the correlation test, ST4 was in a negative line regression with Kd and a positive one with Bmax. Mitochondrial ATP content in hypoxia group decreased to 58.4% of control group (P0.01). The membrane potential was decrease to 80% of control group (P0.01). Conclusion Hypoxia can increase the content and activity of brain mitochondrial UCP that affects mitochondrial respiratory oxygen consumption and energy production.
【Fund】: 全军医学科学技术研究“十一五”计划课题(06MA193)~~
【CateGory Index】: R363
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved