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《Acta Academiae Medicinae Militaris Tertiae》 2006-21
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Growth properties of cultured dermal papilla cells from human hair follicles

GUO Xiao-jing~1, HAO Fei~1, JIANG Xiao-yong~2, SONG Zhi-qiang~1 (~1Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 400038, ~2Department of Dermatology, Zhongshan Hospital of Xiamen University, Xiamen 361004, China)  
Objective To observe the growth properties of dermal papilla cells from human hair follicles and accumulate data for hair reconstruction. Methods Dermal papillae were isolated from human scalp hair follicles by digestion with dispase and collagenase D and cultured. The expressions of growth factors released by DPC of different passages were observed by means of immunohistochemistry. Results The high passage DPC lost aggregative growth property, and expressed fewer growth factors and less in amount gradually. Conclusion The growth and properties of DPC were affected by many factors. The cultured DPC is different from DPC in vivo. The conditioned medium for low passage DPC can resume some properties of DPC.
【Fund】: 国家自然科学基金资助项目(30200249)~~
【CateGory Index】: R329
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