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《Acta Academiae Medicinae Militaris Tertiae》 2006-24
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Effect of early wound fluids on proliferation and migration of dermal pluripotent stem cells

QU Ji-fu 1,2, JIANG De-peng1, ZHENG Huai-en2, HAO Li2, WEN Liang1, CHENG Tian-min2 (1Department of Emergency Medicine, Southwest Hospital, 2State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China)  
Objective To explore the effect of dermal pluripotent stem cells on the initiation of wound repairing. Methods Dermal pluripotent stem cells, fibroblasts and vascular endothelial cells were routinely isolated, purified and expanded. Wound fluids of 1 st, 2 nd,3 rd and 4 th day were collected from 12 male Wistar rats to culture the three types of cells mentioned above and their responses to wound fluids were investigated by MTT assay. The abilities of attachment and migration of dermal pluripotent stem cells stimulated by wound fluid of 1 st day were observed. Results The wound fluids could promote the proliferation of dermal pluripotent stem cells, especially the early-phase wound fluids. The responses of fibroblasts and vascular endothelial cells to wound fluids were not so strong as that of dermal pluripotent stem cells. The abilities of attachment and migration of dermal pluripotent stem cells significantly increased after the stimulation of early-phase wound fluids, suggesting that the dermal multipotent stem cells responsed well to wound fluids. Conclusion Dermal pluripotent stem cells may play an important role in the process of wound healing. The significance of dermal pluripotent stem cells in the initiation of wound repair is worth further investigation.
【Fund】: 国家重点基础研究发展规划资助项目(“973”项目)(2005CB522605);; 国家自然科学基金资助项目(30370562)~~
【CateGory Index】: R641
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