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《Acta Academiae Medicinae Militaris Tertiae》 2006-24
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Identification of interaction between HT036 and P311 by co-immunoprecipitation

YUAN Shun-zong 1,2,PENG Xu 1,2,MA Bing 1,2,WANG Qing-hong 1,2,YI Shao-xuan 1,2,HE Wei-feng 1,2,CHEN Xi-wei 1,2,HU Xiao-hong 1,2,ZHANG Xiao-rong 1,2,ZHOU Li-na 1,2,LUO Gao-xing 1,2,WU Jun 1,2(State Key Laboratory of Trauma,Burns and Combined Injury,Institute of Burns,Southwest Hospital,Third Military Medical University,2Chongqing Key Lab for Medical Proteomics,Chongqing 400038,China)  
Objective To explore the interaction between HT036(hypothetical protein HT036)and P311 by co-immunoprecipitation.Methods HA-tagged fusion protein(HA-HT036)expression vector was constructed,identified and transfected into human embryo kidney 293(HEK293)cells alone or with Myc-tagged fusion protein(Myc-P311)expression vector pCMV-Myc-p311.The interaction between P311 and HT036 was detected by co-immunoprecipitation.Results Double restriction enzyme digestion showed that pCMV-HA-HT036 was constructed correctly.When Myc-P311 was immunoprecipitated by anti-Myc antibody,HA-HT036 was identified by Western blotting with anti-HA antibody from immunoprecipitated complex.Conclusion The recombinant vector pCMV-HA-HT036 was constructed successfully.The interaction between HT036 and P311 could be identified by co-immunoprecipitation after co-expression of pCMV-HA-HT036 and pCMV-Myc-p311.The result provides an important basis for further study of the intracellular signal transduction of P311.
【Fund】: 国家自然科学基金资助面上项目(30571922)~~
【CateGory Index】: Q78
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