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《Acta Academiae Medicinae Militaris Tertiae》 2006-24
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Clone of differentially expressed genes of human small-cell lung cancer multi-drug resistance cell line

LI Kun-lin,WU Guo-ming,JI Fu-yun,XU Zhi,HUANG Gui-jun(Institute of Respireatory Diseases,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China)  
Objective To build the subtracted cDNA library of differentially expressed genes of small cell lung cancer(SCLC)multidrug resistance(MDR)cells.Methods The tester is SCLC MDR cell H446/CDDP cDNA;the driver is SCLC cell H446 cDNA;SHH and T/A cloning technology were done to build the subtracted cDNA library of differentially expressed genes of SCLC MDR cells.The dot blot hybridization,sequencing and homology analysis were used to obtain differentially expressed cDNA fragments.Results The subtracted cDNA library of differentially expressed genes of SCLC MDR cells H446/CDDP was successfully built and 21 differentially expressed cDNA fgragments of cell H446/CDDP were obtained.Sequencing and homology analysis show that the 21 fragments respectively represent 6 known genes(96%-100% homology).Conclusion Suppression subtractive hybridization(SSH)is an effective method for screening new function genes.The six genes might participate in the formation of the MDR in H446/CDDP.
【Fund】: 国家自然科学基金资助项目(30200119)~~
【CateGory Index】: R734.2
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