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《Acta Academiae Medicinae Militaris Tertiae》 2006-24
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Degradation and cross-presentation of Escherichia coli in macrophage

LIU Na,WU Yu-zhang,ZHOU Jing-ran,ZOU Li-yun,GUO Sheng,WAN Ying(Institute of Immunology,College of Medicine,Third Military Medical University,Chongqing 400038,China)  
Objective To dynamically investigate the cross-presentation and degradation of E.coli.that expresses a fusion protein DsRed tagged OVA epitope antigen in macrophage.Methods A plasmid that contains the expression element of fusion protein DsRed tagged OVA epitope was constructed and expressed in E.coli..The cross-presentation and degradation of the E.coli.in macrophage were dynamically assessed with cytometry by using a MHC class Ⅰ-peptide complex antibody.Results The expression of recombinant protein was confirmed by cytometry by 488 nm excitation.The phagosome containing E.coli.co-localized with endoplasmic reticulum(ER).The phagocytosed E.coli.degraded rapidly in 3-5 h and at same phase the H-2Kb-OVA_ 257-264 complex reached the maximum.Conclusion The E.coli.containing recombinant protein has similar fluorescence with DsRed.ER mediates the phagocytosis of E.coli..The cross-presentation and degradation of E.coli..are mainly completed in 3-5 h after phagocytosis.
【Fund】: 国家自然科学基金资助项目(30400392)~~
【CateGory Index】: R392
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Chinese Journal Full-text Database 1 Hits
1 LIU Na, WAN Ying, ZHOU Jing-ran, ZOU Li-yun, ZHI Yi, GUO Sheng, WU Yu-zhang (Institute of Immunology, PLA, Third Military Medical University, Chongqing 400038, China);Expression and purification of dsRed tagged OVA epitope fusion protein[J];Immunological Journal;2005-05
Chinese Journal Full-text Database 1 Hits
1 XING Wan-jin,ZHAO Yu-hang,REN Shi-chao,BAO Xiao-hong(Department of Biology,College of Life Sciences,Inner Mongolia University,Hohhot 010021,China);Expression and Observation of Red Fluorescent Protein DsRed2 in E.coli and Its Application in College Experimental Teaching[J];Journal of Inner Mongolia University;2008-05
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 GUO Bo,ZHENG Ping,LI Hua,XIE Pei-rong,YANG Fei,WANG Lei,WU Yu-zhang,ZOU Qiang (Institute of Immunology,PLA, Third Military Medical University,Chongqing 400038,China);Study on the influence factors upon the specificity and sensitivity of CTL killing in vitro[J];Immunological Journal;2004-03
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