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《Acta Academiae Medicinae Militaris Tertiae》 2006-24
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Construction and expression of N-terminal mutants of human Eotaxin

ZHOU Jin-chuan 1,ZHU Jin 2(1Department of Otorhinolaryngology,Southwest Hospital,Third Military Medical University,Chongqing 400038;2Department of Biomedical Engineering,Chongqing University of Medical Sciences,Chongqing 400016,China)  
Objective To construct prokaryotic cell expression vector of human Eotaxin mutants.Methods By point mutation,eight amino acid residues in the N-terminal(residues of 3-7)and N-loop(residue 14)regions of Eotaxin were individually mutated to methionine and residue 14 was delleted or methiomine was inserted after the residue 14,and then cloned respectively into prokaryotic cell expression vector-PET30a+.Results Eight N-terminal and N-loop mutants of human Eotaxin and their prokaryotic cell expression vector-PET30a+ were gained.Conclusion The successful construction of prokaryotic cell expression vector of human Eotaxin mutants lays a foundation for their expression and biological activity and for filtering antagonists of CCR3.
【Fund】: 国家自然科学基金资助项目(30200313)~~
【CateGory Index】: Q78
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