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《Acta Academiae Medicinae Militaris Tertiae》 2006-24
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Effect of insulin on proliferation and collagen protein synthesis of rat vascular smooth muscle cells

WANG Xu-kai 1,WANG Yan 2,YANG Cheng-ming 1,FU Chun-jiang 1,ZHANG Ye 1,HE Zuo-yu 3(1Department of Cardiovasology,Institute of Surgery Research,Daping Hospital,Chongqing 400042, 2Department of Moleculer Genetics,College of Medicine,Chongqing 400038, 3Department of Cardiovascular Disenses,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China)  
Objective To understand the role of insulin in the cytobiology of blood vessels.Methods The vascular smooth muscle cells(VSMCs)from SD rat were cultured and respectively treated with 1,10,20,30,40 nmol/L insulin for 24 h or 30 nmol/L insulin for 12,24,36,48 h,or with DMEM solution containing 10% FBS as blank control.DNA synthesis and collagen protein synthesis of rat VSMCs were detected by 3H-TdR incorporation and 3H-proline incorporation.Results Insulin could significantly promote DNA synthesis and collagen protein synthesis of quiescent rat VSMCs in a dose-dependent manner.DNA synthesis and collagen protein synthesis reached the peak when insulin concentration was 40 nmol/L,and showed significant difference at 36 h and 48 h with that of the blank control.Conclusion Insulin could promote proliferation and collagen protein synthesis of cultured rat VSMCs.
【Fund】: 国家自然科学基金资助项目(30170384 30570564)~~
【CateGory Index】: R363
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