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《Acta Academiae Medicinae Militaris Tertiae》 2006-24
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Effect of antisense oligodeoxynucleotides against hTERT on proliferation and apoptosis of human Tca8113 cancer cell

YAO Chen 1,YAO Hua 2,WU Qiu-liang 2,TAN Long-yi 1,FAN Jun 3,SHI Zhang-ying4(1Department of Experimental Diagnosis,Changzheng Hospital,Second Military Medical University,Shanghai 200003;2Department of Stomatology,The First Affiliated Hospital,3Research Institute of Infectious Disease,College of Medicine,Zhejiang University,Hangzhou 310003,4Department of Laboratory Medicine,No.113 Hospital of PLA,Ningbo 315040,China)  
Objective To study the effect of antisense oligodeoxynualeotides against hTERT on proliferation and apoptosis of human tongue Tca8113 cancer cell.Methods Morphology of Tca8113 cells was observed under microscope.The proliferation of Tca8113 cells was detected by MTT assay.Cell cycles and apoptosis were examined by flow cytometry(FCM).DNA of Tca8113 cells was extracted and identified by gel electrophoresis for evidence “DNA ladder”.The data were analyzed by one-way ANOVA of SPSS 11.0 for windows.Results ASODN inhibited the proliferation of Tca8113 cells significantly,as compared with controls(P0.05),in both time-dependent and dose-dependent manner.The cells of control groups kept exponential reproduce ability.FCM analysis and the DNA ladder also showed that cells treated with ASODN arrested in G_1/G_0,the ratio of cells in S period was reduced and apoptosis rate of Tca8113 cells treated with ASODN for 72 h was significantly increased in comparison with control groups(P0.05).No significant difference was found among the control groups.Conclusion Antisense oligodeoxynucleotides against hTERT could inhibit the proliferation of human Tca8113 cancer cell and promote cell apoptosis.
【Fund】: 浙江省中医药科技计划基金资助项目(2004098)~~
【CateGory Index】: R739.8
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 TIAN Feng-Jun1, WANG Zhi-Yong2, MA Jun-Yi3, ZHAO Yun-Xia1, LU Wei11. Department of Respiratory Medicine, The Third Hospital, Hebei Medical University, Shijiazhuang, Hebei, 050051, P.R.China2. Department of Critical Care Medicine, The Third Hospital, Hebei Medical University, Shijiazhuang, Hebei, 050051, P.R.China3. Department of Respiratory Medicine, The second Hospital, Hebei Medical University, Shijiazhuang, Hebei, 050051, P.R.China;Inhibitory Effect of hTERT dsRNA on Telomerase Activity in Lung Carcinoma Cell Line A549[J];Chinese Journal of Cancer;2005-03
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 JING Kang-zhen reviewing,GAO Jian-ping,CHENG Wen checking(Department of Urology,School of Medicine,Second Military Medical University,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 210002,Jiangsu,China);Gene therapy for bladder cancer: past and future[J];Journal of Medical Postgraduates;2007-08
2 WANG Guo-yu1,XIA Wei1,ZHANG Yu-na1,LI Yan2,ZHU Xue-ping1,WANG Ying3,NI Jing1 1.Department of Nuclear Medicine,Shanghai Putuo District People's Hospital,Shanghai 200060,P.R.China 2.Central Laboratory,Shanghai First Maternity and Infant Hospital,Tongji University,Shanghai 200040,P.R.China 3.Department of Nuclear Medicine,Shanghai Xuhui District Central Hospital,Shanghai 200031,P.R.China;Anti-cancer effect of siRNA targeting human telomerase reverse transcriptase in SW480 cells[J];Chinese Journal of Cancer Prevention and Treatment;2011-21
3 HU Wei, HAO Jun-yuan, ZHANG Hui-ying, et al (Department of Biochemistry and Moelecular Biology, College of Life Science, Jilin Agriculture University, Changchun 130118, China);Induction of Tumor Cell Apoptosis by HSV-TK Suicide Gene System under Control of hTERT Core Promoter[J];Chinese Journal of Biologicals;2008-09
4 Zhao Jiao Yu Hong Zhao Xiaoping et al Department of Biology and Genetics,Luzhou Medical College (Luzhou 646000);Effect evaluation of siRNA expression vector to hTERT[J];Shaanxi Medical Journal;2009-01
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Tian Feng Jun1, Wang Zhi Yong2, Ma Jun Yi3, Zhao Yun Xia1, Lu Wei1 1.Department of Respiratory Disease, The Third Hospital of Hebei Medical University, Shijiazhuang,Hebei,050051, P.R.China 2.Department of Emergency, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050051, P.R.China 3.Department of Respiration, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050051, P.R.China;Inhibition of Anti-sense Human Telomerase Reverse Transcriptase (hTERT) Retroviral Vector on Lung Cancer Cells[J];Chinese Journal of Cancer;2004-05
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