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《Acta Academiae Medicinae Militaris Tertiae》 2006-24
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Role of cGMP-dependent protein kinase against ethanol neurotoxicity of cerebellar granule cells

DAI De(Department of Otolaryngology,The Affiliated Hospital of Guangdong Medical College,Zhanjiang 524001,China)  
Objective To analyze the function of cGMP-dependent protein kinase(PKG)in the survival of cultured rat cerebellar granule cells(CGC).Methods CGC culture was incubated for 24 h before addition of ethanol and different reagents,such as Deta-NONOate(a NO donor),Br-cGMP and Rp-8-pCPT-cGMPS(a cGMP-dependent protein kinase inhibitor).After the treatment,samples were incubated for another 24 h,then the cells were collected and counted by using hemocytometer.Results Deta-NONOate and Br-cGMP enhanced the cell survival in the absence of ethanol,indicating their neurotrophic effects.They showed neuroprotective effect on the cultured CGC since they could reduce the ethanol-induced cell death.Inhibiting PKG with Rp-8-pCPT-cGMPS could eliminate both neurotrophic and neuroprotective effects mediated by Deta-NONOate and Br-cGMP.Conclusion cGMP-dependent protein kinase plays a key role in the cultured CGC survival and protects the cells against ethanol neurotoxicity.
【Fund】: 湛江市科学技术局科研基金资助项目(2006C01021)~~
【CateGory Index】: R363
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