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《Acta Academiae Medicinae Militaris Tertiae》 2006-24
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Expression of functional formylpeptide receptor FPR by primary human malignant glioma cells

FENG Yu-hui,BIAN Xiu-wu,GUO De-yu,ZHAO Wen,LIU Long,CHEN Jian-hong(Department of Pathology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China)  
Objective To detect the expression of formylpeptide receptor FPR in primary human glioma cells and its functional activity when stimulated with the ligand fMLF.Methods Human glioma tissues were employed with or without digestion with trypsin for primary culture of glioma cells.By using indirect immunofluorescence and confocal laser scanning microscopy,the expression and localization of FPR in the primary glioma cells were detected.Meanwhile,the cell proliferation in vitro and the production of vascular endothelial growth factor(VEGF)by primary cultured cells after FPR activation with its ligand——fMLF,were assessed by trypan blue exclusion assay and enzyme-linked immunosorbent assay(ELISA),respectively.Results The primary human malignant glioma cells expressed FPR on the plasma membrane,presenting discontinuous linear fluorescence.After FPR activation by fMLF,the cell proliferation in vitro was promoted,and the VEGF level secreted by primary glioma cells was increased significantly.A significantly positive correlation was found between FPR expression and the production of VEGF.Conclusion FPR was selectively expressed by the primary malignant glioma cells,and FPR activation promoted cell proliferation and the production of angiogenic factor VEGF in vitro.
【CateGory Index】: R739.41
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